山东科学 ›› 2018, Vol. 31 ›› Issue (5): 38-42.doi: 10.3976/j.issn.1002-4026.2018.05.007

• 生物传感器 • 上一篇    下一篇

乳酸菌发酵过程参数的研究

杨俊慧1,马恒1,满德恩2,郭脉海2,刘庆艾1,马耀宏1,史建国1*   

  1. 1.齐鲁工业大学(山东省科学院),山东省科学院生物研究所,山东省生物传感器重点实验室,山东 济南 250014;2.菱花集团有限公司,山东 济宁 272032
  • 收稿日期:2018-04-04 出版日期:2018-10-20 发布日期:2018-10-20
  • 通信作者: 史建国(1960—),男,研究员,研究方向为微生物学。E-mail: shijg@sdas.org E-mail:shijg@sdas.org
  • 作者简介:杨俊慧(1969—),女,高级实验师,研究方向为生物学。E-mail: swdz@sdas.org
  • 基金资助:

    山东省自然科学基金中青年科学家科研奖励基金(ZR2016CB42);山东省重点研发计划(2017GSF221014);山东省科学院青年科学基金(2015QN009)

Study on the fermentation parameters of lactic acid bacteria

YANG Jun-hui1, MA Heng1, MAN De-en2, GUO Mai-hai2, LIU Qing-ai1, MA Yao-hong1, SHI Jian-guo1*   

  1. Shandong Academy of Sciences), Jinan 250014, China;2. Linghua Group Limited,Jining 272032,China
  • Received:2018-04-04 Online:2018-10-20 Published:2018-10-20

摘要:

为准确、快捷地掌握乳酸菌发酵过程中主要参数的变化情况,通过KRH-BI0300型发酵罐控制系统、细胞密度监测系统、SHP8400PM过程气体质谱分析仪、生物传感分析仪等多种发酵过程分析检测手段,对乳酸发酵过程中乳酸菌活细胞数、尾气成分、葡萄糖及乳酸含量的变化情况进行测定。结果表明,应用多种现代化分析检测手段可以快速、精确地掌握乳酸菌发酵过程参数变化情况。该研究为乳酸菌发酵过程的优化控制及规模工业化奠定了基础。

关键词: 乳酸菌发酵, 过程参数, 检测方法

Abstract:

In order to accurately and quickly grasp the changes of the main parameters in the fermentation process of lactic acid bacteria, the changes in the number of viable cells, tail gas components, glucose and lactic acid in the lactic acid fermentation process were measured by KRH-BI0300 type fermentation tank control system, cell density monitoring system, SHP8400PM process gas mass spectrometry analyzer, biological sensor analyzer and other fermentation process analysis methods. The results show that the parameters of lactic acid bacteria fermentation process can be quickly and accurately grasped by a variety of modern analytical methods. This study laid the foundation for optimal control and lactic acid bacteria industrialization fermentation process.

Key words: detection method, process parameters, lactic acid bacteria fermentation

中图分类号: 

  • Q815