氯霉素对斑马鱼幼鱼发育及免疫毒性的研究

doi:10.3976/j.issn.1002-4026.2017.06.006

Abstract

Abstract:

The impact of chloramphenicol on developmental toxicity and immune cells of wild and transgenic Tg(lyz:EGFP) juvenile zebrafish was investigated. After treated separately with different concentrations of chloramphenicol for 72 hours, the survival rate, abnormal rate, morphology variation of juvenile zebrafish, and the number of immune cells labeled with green fluorescent in juvenile zebrafish were observed. The results showed that with the increase of the concentration of

chloramphenicol (≥100 mg/L) and the exposure time, the survival rate and hatching rate of zebrafish decreased, and mortality increased. The teratogenic effects of chloramphenicol on juvenile zebrafish included yolk sac edema, pericardial edema and spinal curvature. Through observation of the transgenic strain Tg(lyz:EGFP), it was found that chloramphenicol (≥75 mg/L) could inhibit the number of immune cells in zebrafish. This study showed that chloramphenicol had teratogenic and lethal effects on juvenile zebrafish in a time dependent and dose dependent manner. And chloramphenicol had immune toxicity, which could reduce the number of immune cells in juvenile zebrafish.

Key words: zebrafish, immune cell, developmental toxicity, chloramphenicol

CLC Number:

R737.33

WANG Rong-chun，HE Qiu-xia，HAN Jian，CHEN Xi-qiang，SUN Chen，WANG Xi-min，HAN Li-wen，LIU Ke-chun. Research on the effects of chloramphenicol on developmental and immune toxicity of juvenile zebrafish[J].SHANDONG SCIENCE, 2017, 30(6): 35-40.

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Research on the effects of chloramphenicol on developmental and immune toxicity of juvenile zebrafish

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