利用斑马鱼模型研究芦笋有效成分对免疫功能的调节作用

doi:10.3976/j.issn.1002-4026.2017.06.005

Abstract

Abstract:

In this experiment, 48 hpf (hour post fertilization) healthy transgenic zebrafish Tg(lyz: EGFP) was used as animal model. Firstly, immune suppression of zebrafish embryos was induced by chloramphenicol treatment. Then those zebrafish embryos were treated with 25.0~200.0 mg/L asparagus polysaccharide and 6.25~100.00 mg/L asparagus saponins for 24 h, respectively. Finally, the development of zebrafish in each group was observed under the microscope,and the number of neutrophils in zebrafish was recorded and counted to evaluate their immuneregulatory effects. The results showed that the numbers of neutrophils in 72hpf zebrafish trunk area decreased in chloramphenicolinduced group.Asparagus polysaccharide group(50.0~200.0 mg/L) showed a significant immunological enhancement effect with dosedependent manner. Asparagus saponins could also significantly improve chloramphenicolinduced immunosuppressive effects within the experimental dose range. In this study, This study showed that the active ingredients of asparagus, such as polysaccharide and asparagus saponin, had obvious regulation effects on the immune function of zebrafish, which provided the scientific basis for further development of asparagus.

Key words: Asparagus, Asparagus saponins, zebrafish, Asparagus polysaccharide, immune-regulation

CLC Number:

R965.1

LIU Fa-sheng, WANG Rong-chun, GUO Jing-lan, CHEN Yan-li, XIA Qing, KONG Hao-tian, FENG Run-liang, HAN Li-wen, ZHANG Ai-ping, LIU Ke-chun. The immune-regulatory effects of active ingredients of asparagus using zebrafish model[J].SHANDONG SCIENCE, 2017, 30(6): 29-34.

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The immune-regulatory effects of active ingredients of asparagus using zebrafish model

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