Abstract:

In this paper, the flavonoids extraction rate was taken as the index, the effects of ethanol concentration, dosage, extraction times and extraction time on the extraction results were investigated, and through BoxBehnken design, the response surface model was established to optimize the parameters of the technology for extracting total flavonoids from Bidens bipinnata L. stems. The results showed that the optimum extraction conditions of total flavonoids from Bidens bipinnata L. stems. were as follows: the ethanol volume fraction was 70%, liquidsolid ratio was12∶1(mL/g), extracted 3 times, and the extraction time was 1.5 h. In these conditions, the maximum extraction rate of total flavonoids could reach 0.95%, and the relative error was 3.06% when compared with the theoretical value of 0.98%.

Key words: Bidens bipinnata L., extraction, the response surface methodology, total flavonoids