利用斑马鱼模型研究芦荟大黄素对宫颈癌细胞SiHa的抑制作用

doi:10.3976/j.issn.1002-4026.2016.02.004

Abstract

Abstract:

We employ MTT to investigate inhibitory effect of different concentrations of aloe emodin (AE) on the proliferation of cervical cancer cell SiHa. We further establish zebrafish xenograft model by injecting SiHa into the yolk sac of 48 hpf zebrafish. The zebrafish is then treated with different concentrations of AE to investigate proliferation and migration of cancer cell in zebrafish. Results show that growth inhibition rate of AE on SiHa significantly increases with the increase of AE concentration and action time, a certain concentrationtimeeffect dependence. In vivo experiment shows that AE has inhibitory effect on the proliferation and metastasis of zebrafish xenograft. Our research indicates that AE has the potential of cervical anticancer drug. The zebrafish xenograft model can also be an important model of cervical cancer research and its drug assessment.

Key words: zebrafish, cervical cancer cell, xenograft model, SiHa, aloe emodine

CLC Number:

R965.1

WANG Rongchun，HAN Liwen，CHEN Xiqiang，HE Qiuxia，WANG Ximin，PENG Weibing，SUN Chen，HAN Jian，LIU Kechun. Inhibitory effect of aloe emodine on cervical cancer cell line SiHa with zebrafish xenograft model[J].SHANDONG SCIENCE, 2016, 29(2): 14-18.

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Inhibitory effect of aloe emodine on cervical cancer cell line SiHa with zebrafish xenograft model

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