山楂叶提取物中牡荆素、金丝桃苷、异槲皮苷对照品的制备

doi:10.3976/j.issn.1002-4026.2017.04.003

Abstract

Abstract:

Three low content flavonoids, such as vitexin, hyperoside and isoquercitrin from Crataegi Folium were rapidly separated and purified by highspeed countercurrent chromatography (HSCCC) combined with preHPLC. The total flavonoids from Crataegi Folium were firstly separated by HSCCC, with a solvent system of chloroform/methanol/water/ nbutanol (4∶3∶2∶1.5, V/V), flowrate of 5.0 mL/min, rotation speed of 850 r/min, detection wavelength of 254 nm. A mixed component was separated from crude flavonoids, containing vitexin, hyperoside, and isoquercitrin. Then, the mixture was separated by preHPLC and vitexin, hyperoside, and isoquercitrin were obtained, with the purity over 98%, respectively, as determined by HPLC. Experimental results show that it is an efficient method for separation of low content flavonoids with high purities from Crataegi Folium by HSCCC combined with pre-HPLC.

Key words: isoquercitrin, pre-HPLC, Crataegi Folium, HSCCC, vitexin, hyperoside

CLC Number:

R284.2

WEN Lei, LIN Yun-liang, GAO Hong-mei, LI Feng, JIANG Jiao-jiao, SONG Xiang-yun, FU Rui-ming, WANG Dai-jie. Preparation of reference substances of vitexin, hyperoside and isoquercitrin from Crataegi Folium extract[J].SHANDONG SCIENCE, 2017, 30(4): 13-18.

References

［1］国家中医药管理局中华本草编委会. 中华本草(第四卷)［M］. 上海: 上海科学技术出版社, 1999: 126.
［2］叶希韵, 程容懿, 徐敏华, 等. 山楂叶总黄酮防治鹌鹁动脉粥状硬化［J］. 华东师范人学学报(自然科学版), 2003(2): 106109.
［3］叶希韵, 张隆, 张静, 等. 山楂叶总黄酮对乳鼠心肌细胞缺血缺氧损伤的实验研究［J］. 中国现代应用药学杂志, 2005, 22(3): 202204.
［4］叶希韵, 张隆, 沈菊, 等. 山楂叶总黄酮对糖尿病小鼠糖脂代谢的影响［J］. 中草药, 2005, 36(11): 16831686.
［5］权迎春, 关丽萍. 山楂叶提取物的抗炎与镇痛作用研究［J］. 时珍国医国药, 2006, 17(4): 556557.
［6］国家药典委员会. 中华人民共和国药典2015年版一部［M］. 北京: 中国医药科技出版社, 2015: 32, 396, 506, 1402.
［7］ZHANG P C, XU S X. Flavonoid ketohexosefuranosides from the leaves of Crataegus pinnatifida Bge. var. major N.E.Br.［J］. Phytochemistry, 2001, 57(8): 12491253.
［8］ ZHANG P C, XU S X. Cglucoside flavonoids from the leaves of Crataegus pinnatifida Bge. var. major N.E.Br.［J］. Asian Nat Prod Res, 2003, 5(2): 131136.
［9］ ZHANG P C, XU S X. Two new Cglucoside flavonoids from leaves of Crataegu spinnatifida Bge. var. major N.E.Br.［J］. Chin Chem Lett, 2002, 13(4): 337340.
［10］ ZHANG P C, XU S X. Chemical constituents from the leaves of Crataegus pinnatifida Bge. Var. major N.E.Br.［J］. Acta Pharm Sin, 2001, 36(10): 754757.
［11］ SVEDSTRM U, VUORELA H, KOSTIAINEN R, et al. Isolation and identification of oligomeric procyanidins from Crataegus leaves and flowers［J］. Phytochemistry, 2002, 60(8): 821825.
［12］ BYKOV V I, GLYZIN V I, BAN’KOVAKII A I. PinnatifidinA new flavonol glycoside from Crataegus pinnatifida［J］. Chem Nat Compd, 1972, 8(6): 699701.
［13］ GAO P Y, LI L Z, PENG Y, et al. Monoterpene and lignan glycosides in the leaves of Crataegus pinnatifida ［J］. Biochem Syst Ecol, 2010, 38(5): 988992.
［14］ HUANG X X, GUO D D, LI L Z, et al. Monoterpene and sesquilignan compounds from the leaves of Crataegus pinnatifida［J］.Biochem Syst Ecol, 2013, 48: 15.
［15］ LIU R M, LI A F, SUN A L, et al. Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by highspeed countercurrent chromatography［J］. J Chromatogr A,2005, 1064(1): 5357.
［16］ CHOO C Y, SULONG N Y, MAN F, et al. Vitexin and isovitexin from the leaves of Ficus deltoidea with invivo αglucosidase inhibition［J］. J Ethnopharmacol, 2012, 142(3): 776781.
［17］ DENG S G, DENG Z Y, FAN Y W, et al. Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by highspeed countercurrent chromatography［J］. J Chromatogr B, 2009, 877(24): 24872492.
［18］LI H J, LIU Y, YI Y T, et al. Purification of quercetin3Osophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH20 column chromatography［J］. J Chromatogr B, 2017, 1048: 5663.

[1]	WU Dan, WANG Jing, Huang Bi-yun, WANG Yan, LIU Long. Simultaneous determination of six components in Bidens parviflora Willd. by UPLC-MS/MS [J]. Shandong Science, 2019, 32(3): 16-22.
[2]	WU Qiu-xia, SUN Chang-lei, WANG Xiao, ZHOU Jie, ZHENG Zhen-jia, YANG Bing-tian, SUN Wei-hong. Separation and preparation of the chemical components of Rheum officinale Baill.by pH-zone-refining counter-current chromatography and HSCCC [J]. Shandong Science, 2015, 28(1): 1-6.
[3]	LIU Hao, HANG Wei, ZHOU Xiao-Jing, CHEN Yi-Lun, WANG Xiao, DUAN Wen-Juan. Separation and preparation of paeoniflorin from Paeonia lactiflora Pall. by HSCCC with salt solution system [J]. J4, 2014, 27(3): 12-16.
[4]	LIU Hong-Yan. HPLC based simultaneous three flavonoid contents determination for Bidens parviflora Willd. [J]. J4, 2014, 27(2): 44-46.
[5]	LIN Xiao-Jing, GENG Yan-Ling, LIU Feng, FU Mao-Run, LIU Jian-Hua, WANG Xiao. Separation and purification of imperatorin and isoimperatorin with supercritical CO₂ fluid extraction high-speed counter-current chromatography [J]. Shandong Science, 2011, 24(6): 39-43.

Preparation of reference substances of vitexin, hyperoside and isoquercitrin from Crataegi Folium extract

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0