Abstract:

      We established a method of separation and preparation of paeoniflorin from Paeonia lactiflora Pall. by highspeed counter current chromatography (HSCCC) with salt solution system. The separation was performed with butanolethyl acetate5%Na2SO4 (aq) (4:1:5,V/V) as a twophase solvent system, flow rate of 2.0 mL/min, rotation number of 800 r/min and detection wavelength of 254 nm. We obtained paeoniflorin of 56.13 mg from the crude extracts of 100 mg at one time. Its purity was more than 98%, which was determined by high performance liquid chromatography（HPLC）. The structure of the target compound was identified by electrospray ionisation mass spectrometry (ESIMS),1Hnuclear magnetic resonance (¹H-NMR) and ¹³C-nuclear magnetic resonance (¹³C-NMR). The method is simple and reproducible and suitable for separation and preparation of paeoniflorin.

Key words: Paeonia lactiflora Pall., HSCCC, Na₂SO₄ (aq), paeoniflorin