Abstract:

We established a HPLC method for content determination of puerarin, daidzin and icariin in Fugu Huangtong Dispersible Tablets. It involves Eclipse XDB-C₁₈ column (4.6 mm×250 mm,5 μm), mobile phase acetonitrilewateracetic acid solution with flow rate of 0.8 mL·min1 and column temperature of 25 ℃, detection wavelength of 250 nm for puerarin and daidzin, and detection wavelength of 270 nm for icariin. Experimental results show that puerarin has better linear relationship with peak area in the range of 0.002 08 mg/mL～0.104 mg/mL, recovery rate of 100.29% and relative standard deviation of 0.89%. Daidzin has better linear relationship with peak area in the range of 0.000 98 mg/mL～0.039 2 mg/mL, recovery rate of 98.29% and relative standard deviation of 1.29%. Icariin has better linear relationship with peak area in the range of 0.001 04 mg/mL～0.041 6 mg/mL, recovery rate of 99.07% and relative standard deviation of 1.08%. The method is of simplicity, accuracy, good reproducibility and stability, so it can be applied to quality control of Fugu Huangtong Dispersible Tablets.

Key words: Fugu Huangtong Dispersible Tablets, icariin, daidzin, HPLC, puerari