一株菲降解菌的分离、鉴定及最佳培养条件的优化

doi:10.3976/j.issn.1002-4026.2015.02.013

Abstract

Abstract: A phenanthrene degradable strain SP3 was isolated from petrolcontaminated soil. It was roughly identified as Mycobacterium sp.through physiological and biochemical identification and contrast analysis of 16S rDNA sequences. Phenanthrene could be served as its unique carbon source.The degradation rate is more than 99% in such conditions as mineral salt medium of phenanthrene concentration of 100 μg/mL,shaking culture of 28 ℃ and 7 days. We discovered that optimal culture medium of strain SP3 included starch of 1%, beef extract of 1%,Na2HPO4 of 0.2%and NaH2PO4 of 0.2%. We determined its optimum culture conditions of temperature of 30 ℃, initial pH of 7.5 and culture time of 12 h through single factor experiment.

Key words: optimization of medium, degradation, phenanthrene, PAHs

CLC Number:

HUANG Yujie, QIU Weizhong, GAO Yongchao, KONG Xue, ZHANG Wen, WANG Jianing. Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria[J].SHANDONG SCIENCE, 2015, 28(2): 75-80.

References

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Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria

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