基于多技术联用的山东省不同产地金银花化学成分差异性分析

doi:10.3976/j.issn.1002-4026.2025170

Abstract

Abstract: Lonicerae Japonicae Flos (LJF) is a traditional Chinese medicine known for its heat-clearing and detoxifying properties, with Shandong Province being a major production area. However, studies on differences in chemical composition among different regions within Shandong (especially saline-alkali lands) are limited. Furthermore, a single analytical technique is insufficient to comprehensively cover volatile and nonvolatile key components, thereby limiting origin traceability and quality evaluation. In this study, 13 batches of genetically similar LJF samples from four regions in Shandong—Guangrao (Dongying), Yuncheng (Heze), Yinan (Linyi), and Pingyi (Linyi)—were analyzed using a combination of three techniques, i.e., headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS), headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS), and ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Chemometric methods including one-way analysis of variance and orthogonal partial least squares–discriminant analysis (OPLS-DA) were employed to analyze compositional differences. A total of 267 compounds were identified, including five terpenoids unique to Guangrao, which are associated with saline-alkali stress. HS-GC-IMS detected 54 volatile compounds, with aldehydes, ketones, alcohols, and esters accounting for 87.0% of the total. HS-SPME-GC-MS analysis identified 16 differential components, nine of which were aldehydes. UPLC-Q-TOF-MS analysis identified 13 differential components. The OPLS-DA model demonstrated good stability (R²_Y > 0.95, Q² > 0.89). This multitechnique approach provides a scientific basis for the quality evaluation and traceability of LJF in Shandong.

Key words: Lonicerae Japonicae Flos, different regions, ion mobility spectrometry, headspace solid-phase microextraction, ultra-performance liquid chromatography, chemometrics

CLC Number:

O657

NING Xiaobei, LU Heng, WANG Xiao, KANG Chuanzhi, JI Wenhua. Analysis of differences in the chemical composition of Lonicerae Japonicae Flos from different regions in Shandong Province based on combined multitechniques[J].Shandong Science, 0, (): 1-.

References

[1] 钱桂英, 钱云英, 吴晓明, 等. 超高效液相色谱-串联质谱结合化学计量学分析金银花中多组分含量[J]. 山东科学, 2023, 36(5): 1-8. DOI:10.3976/j.issn.1002-4026.2023.05.001.

[2] Cao Yuxia, Ji Peng, Wu Fanlin, et al.Lonicerae Japonicae Caulis: A review of its research progress of active metabolites and pharmacological effects[J]. Frontiers in Pharmacology, 2023, 14: 1277283. DOI:10.3389/fphar.2023.1277283.

[3] Li Yuke, Xie Long, Liu Kai, et al. Bioactive components and beneficial bioactivities of flowers, stems, leaves ofLonicerajaponicaThunberg: A review[J]. Biochemical Systematics and Ecology, 2023, 106: 104570. DOI:10.1016/j.bse.2022.104570.

[4] 李万英, 王海燕, 汪慧铖, 等. 响应面法优化金银花中有机酸类成分提取工艺[J]. 山东科学, 2025, 38(4): 1-7. DOI:10.3976/j.issn.1002-4026.20240053.

[5] 郑婷月, 刘文静, 董树清, 等. 金银花挥发油提取、成分分析及药理作用的研究进展[J]. 华西药学杂志, 2025, 40(3): 331-337. DOI:10.13375/j.cnki.wcjps.2025.03.020.

[6] 王丽, 胡付侠, 张明, 等. 蒸汽爆破辅助超声提取金银花绿原酸及其抗氧化、抗炎活性研究[J]. 食品科技, 2025, 50(3): 225-234. DOI:10.13684/j.cnki.spkj.2025.03.007.

[7] Li Qianqian, Zhang Jialin, Lin Tao, et al. Migration behavior and dietary exposure risk assessment of pesticides residues in honeysuckle (LonicerajaponicaThunb.) based on modified QuEChERS method coupled with tandem mass spectrometry[J]. Food Research International, 2023, 166: 112572. DOI:10.1016/j.foodres.2023.112572.

[8] 马涵玉, 钱琪, 王凤霞, 等. 基于HS-GC-MS和电子感官技术比较不同产地金银花气味和味道差异[J]. 中草药, 2024, 55(6): 2085-2093. DOI:10.7501/j.issn.0253-2670.2024.06.030.

[9] 程喜真, 张玉玲, 郭浩川, 等. 不同产地金银花初生代谢物化学成分的GC-MS分析[J]. 武汉大学学报(理学版), 2024, 70(2): 229-235. DOI:10.14188/j.1671-8836.2023.0107.

[10] 刘璐, 吕凌, 李晨, 等. 基于多指标成分和模式识别分析不同产地金银花的质量差异[J]. 中华中医药杂志, 2023, 38(9): 4328-4335.

[11] 寿璐佳, 赵晨阳, 颜世利, 等. GC-MS指纹图谱结合化学计量学分析不同产地金银花挥发油的成分变化[J]. 沈阳药科大学学报, 2022, 39(8): 913-918. DOI:10.14066/j.cnki.cn21-1349/r.2021.1401.

[12] 王玲娜, 苏征, 刘星劼, 等. 金银花活性成分与生态因子相关性研究[J]. 中国实验方剂学杂志, 2016, 22(17): 27-31. DOI:10.13422/j.cnki.syfjx.2016170027.

[13] 韩赟, 谷巍, 沈夕坤, 等. 不同产地金银花及其掺杂叶中活性成分比较研究[J]. 南京中医药大学学报, 2017, 33(1): 87-92. DOI:10.14148/j.issn.1672-0482.2017.0087.

[14] 蔡芷辰, 陈欢, 杨蓉, 等. 基于液相色谱-串联质谱结合多元统计分析盐胁迫下金银花代谢产物积累的变化[J]. 南京中医药大学学报, 2020, 36(5): 584-592. DOI:10.14148/j.issn.1672-0482.2020.0584.

[15] 鞠龙泰, 焦焕然, 孙盼盼, 等. 金银花受环境胁迫影响研究进展[J]. 北方园艺, 2021(21): 136-142. DOI:10.11937/bfyy.20202907.

[16] Wang Shuqi, Chen Haitao, Sun Baoguo. Recent progress in food flavor analysis using gas chromatography–ion mobility spectrometry (GC–IMS)[J]. Food Chemistry, 2020, 315: 126158. DOI:10.1016/j.foodchem.2019.126158.

[17] Rong Yuting, Xie Jialing, Yuan Haibo, et al. Characterization of volatile metabolites in Pu-erh teas with different storage years by combining GC-E-Nose, GC-MS, and GC-IMS[J]. Food Chemistry, 2023, 18: 100693. DOI:10.1016/j.fochx.2023.100693.

[18] Bi Jicai, Li Yang, Yang Zhen, et al. Effect of different cooking times on the fat flavor compounds of pork belly[J]. Journal of Food Biochemistry, 2022, 46(8): e14184. DOI:10.1111/jfbc.14184.

[19] Yin Jiaxin, Wu Mengfan, Lin Ruimei, et al. Application and development trends of gas chromatography–ion mobility spectrometry for traditional Chinese medicine, clinical, food and environmental analysis[J]. Microchemical Journal, 2021, 168: 106527. DOI:10.1016/j.microc.2021.106527.

[20] Li Weidan, Li Junjie, Qin Zhen, et al. Insights into the composition and antibacterial activity ofAmomumtsao-ko essential oils from different regions based on GC-MS and GC-IMS[J]. Foods, 2022, 11(10): 1402. DOI:10.3390/foods11101402.

[21] 陈臣, 田同辉, 刘政, 等. 基于感官评价、GC-IMS和GC-MS的中式酸凝奶酪挥发性风味比较[J]. 食品科学, 2023, 44(16): 228-236. DOI:10.7506/spkx1002-6630-20221013-124.

[22] Wang Qiao, Du Baoquan, et al. Saline-alkali stress affects the accumulation of proanthocyanidins and sesquiterpenoidsviathe MYB5-ANR/TPS31 cascades in the rose petals[J]. Horticulture Research, 2024, 11(11): uhae243. DOI:10.1093/hr/uhae243.

[23] Mansinhos I, Gonçalves S, Romano A. How climate change-related abiotic factors affect the production of industrial valuable compounds in Lamiaceae plant species: A review[J]. Frontiers in Plant Science, 2024, 15: 1370810. DOI:10.3389/fpls.2024.1370810.

[24] Zhang Mingzhu, Guo Dongfeng, Wang Haiqing, et al. Evaluation of microbial and organic metabolite characteristics in fermented tobacco from different grades by comprehensive microbiomes and chemometrics analysis[J]. Industrial Crops and Products, 2024, 222: 119706. DOI:10.1016/j.indcrop.2024.119706.

[25] Vincenti S, Mariani M, Alberti J C, et al. Biocatalytic synthesis of natural green leaf volatiles using the lipoxygenase metabolic pathway[J]. Catalysts, 2019, 9(10): 873. DOI:10.3390/catal9100873.

[26] Jo H E, Song K, Kim J G, et al. Non-targeted metabolomic analysis for the comparative evaluation of volatile organic compounds in 20 globally representative cucumber lines[J]. Frontiers in Plant Science, 2022, 13: 1028735. DOI:10.3389/fpls.2022.1028735.

[27] He Lei, Xu Xiaoqing, Wang Yu, et al. Modulation of volatile compound metabolome and transcriptome in grape berries exposed to sunlight under dry-hot climate[J]. BMC Plant Biology, 2020, 20(1): 59. DOI:10.1186/s12870-020-2268-y.

[28] Zhou Wanhai, Kong Wenbin, Yang Can, et al. Alcohol acyltransferase is involved in the biosynthesis of C6 esters in apricot (Prunus armeniacaL.) fruit[J]. Frontiers in Plant Science, 2021, 12: 763139. DOI:10.3389/fpls.2021.763139.

[29] Meethaworn K, Luckanatinwong V, Zhang Bo, et al. Off-flavor caused by cold storage is related to induced activity of LOX and HPL in young coconut fruit[J]. LWT, 2019, 114: 108329. DOI:10.1016/j.lwt.2019.108329.

[30] Shen Jiyuan, Wu Lei, Liu Hongru, et al. Bagging treatment influences production of C6 aldehydes and biosynthesis-related gene expression in peach fruit skin[J]. Molecules, 2014, 19(9): 13461-13472. DOI:10.3390/molecules190913461.

[31] Padilla M N, Hernández M L, Sanz C, et al. Stress-dependent regulation of 13-lipoxygenases and 13-hydroperoxide lyase in olive fruit mesocarp[J]. Phytochemistry, 2014, 102: 80-88. DOI:10.1016/j.phytochem.2014.01.024.

[32] Vieira D D S S, Emiliani G, Michelozzi M, et al. Polyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (CitruslimoniaOsb.) leaves[J]. Environmental and Experimental Botany, 2016, 126: 1-9. DOI:10.1016/j.envexpbot.2016.02.010.

[33] Wang Yan, Li Lili, Ji Wenhua, et al. Metabolomics analysis of different tissues ofLonicera japonicathunb. Based on liquid chromatography with mass spectrometry[J]. Metabolites, 2023, 13(2): 186. DOI:10.3390/metabo13020186.

[34] Zhou Huapeng, Shi Haifan, Yang Yongqing, et al. Insights into plant salt stress signaling and tolerance[J]. Journal of Genetics and Genomics, 2024, 51(1): 16-34. DOI:10.1016/j.jgg.2023.08.007.

[35] Duan Yifan, Jiang Liqiong, Lei Ting, et al. Increasing Ca2+accumulation in salt glands under salt stress increases stronger selective secretion of Na+inPlumbago auriculatatetraploids[J]. Frontiers in Plant Science, 2024, 15: 1376427. DOI:10.3389/fpls.2024.1376427.

[36] Heng Tong, Ma Yingjie, Ai Pengrui, et al. The effects of soil salt stress on the nitrogen uptake, yield response and nitrogen use efficiency of cotton in arid areas[J]. Agronomy, 2024, 14(1): 229. DOI:10.3390/agronomy14010229.

[37] Sarker U, Oba S. Salinity stress enhances color parameters, bioactive leaf pigments, vitamins, polyphenols, flavonoids and antioxidant activity in selectedAmaranthusleafyvegetables[J]. Journal of the Science of Food and Agriculture, 2019, 99(5): 2275-2284. DOI:10.1002/jsfa.9423.

[38] Šamec D, Linić I, Salopek-Sondi B. Salinity stress as an elicitor for phytochemicals and minerals accumulation in selected leafy vegetables of Brassicaceae[J]. Agronomy, 2021, 11(2): 361. DOI:10.3390/agronomy11020361.

[39] Sarker U, Oba S. Augmentation of leaf color parameters, pigments, vitamins, phenolic acids, flavonoids and antioxidant activity in selectedAmaranthustricolorunder salinity stress[J]. Scientific Reports, 2018, 8: 12349. DOI:10.1038/s41598-018-30897-6.

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Analysis of differences in the chemical composition of Lonicerae Japonicae Flos from different regions in Shandong Province based on combined multitechniques

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