SHANDONG SCIENCE ›› 2017, Vol. 30 ›› Issue (1): 15-19.doi: 10.3976/j.issn.1002-4026.2017.01.003

• Traditional Chinese medicine and natural active products • Previous Articles     Next Articles

Separation and purification of glycosidic compounds from  Folium Sennae by high-speed counter-current chromatography

Duan Wen-juan1, WU Qiu-xia1, 2, Li Yue1, Yang Guo-hong1, SHI Xin-gang1, Sun Wei-hong2, WANG Xiao1*   

  1. 1.Shandong Provincial Key Laboratory for TCM Quality Control Technology, Shandong Analysis and test Center, Jinan 250014, China; 2. School of Food and Biology Engineering,  Jiangsu University, Zhenjiang 212013, China
  • Received:2016-11-02 Published:2017-02-20 Online:2017-02-20

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Abstract:

Four glycosidic compounds were rapidly separated from the nbutyl alcohol of Folium Sennae by high-speed counter-current chromatography with the solvent system of nbutanolwater (9:10, V/V). The flow rate is 2.0 mL/min, the detection wavelength is 280 nm, and the rotate speed rating is 860 r/min. Separated from the nbutyl alcohol extracts (200 mg) of Folium Sennae, their structures were identified as apigenin-6, 8-di-C-glycoside (10 mg), kaempferol-3-O-β-D gentiobioside (9 mg), isorhamnetin-3-O-β-D gentiobioside (15 mg), and sennoside B (5 mg) with the purities were respectively 97.6%, 98.9%, 98.3%, and 99.3%. The results show that this method is simple and efficient, which is suitable for the separation and purification of glycosidic compounds from Folium Sennae.

Key words: highspeed countercurrent chromatography, separation and purification, Folium Sennae, glycosidic compounds

CLC Number: 

  • R284.2

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