Abstract:

We rapidly separated and purified salvianolic acid B and rosmarinic acid from Salvia miltiorrhiza Bge. By pHzonerefining countercurrent chromatography ( pH-ZRCCC) . The extraction conditions included solvent system of ethyl acetatewater ( 1∶1, V/ V) , upper stationary phase of 10 mmol/ L trifluoroacetic acid, lower mobile phase of 20 mmol/ L NH ₃ H ₂ O, rotation speed of 850 r/ min, flow speed of 2. 0 mL/ min, and detection wavelength of 280 nm. Salvianolic acid B of 386 mg and rosmarinic acid of 25 mg were extractedfrom crude extract of 500 mg, whose purities were 96. 3% and 98.1% through HPLC analysis. Their compound structures were identified by ESIMS and 1 HNMR. Experimental results show that pHZRCCC is a rapid and efficient method to separate and purify salvianolic acid B and rosmarinic acid.

Key words: pH-zone-refining counter-current chromatography')">pH-zone-refining counter-current chromatography, rosmarinic acid, salvianolic acid B