阿特拉津降解细菌的直接检测及分离

doi:10.3976/j.issn.1002-4026.2015.06.004

SHANDONG SCIENCE ›› 2015, Vol. 28 ›› Issue (6): 17-22.doi: 10.3976/j.issn.1002-4026.2015.06.004

• Agricultural Microbiology • Previous Articles Next Articles

Direct detection and isolation of atrazinedegrading bacteria

LI Hongmei, LI Chengyun, LI Jishun, WEI Yanli, YANG Hetong, BAZHANAU Dzmitry

Shandong Provincial Key Laboratory of Applied Microbiology,Biology Institute,Shandong Academy of Sciences, Jinan 250014, China

Received:2015-07-30 Online:2015-12-20 Published:2015-12-20

Abstract

Abstract: We detected, enumerated and isolated atrazinedegrading bacteria from herbicide contaminated soil by improved agar SM. We found that atrazine degraders constituted a major component of microbial communities in industrially contaminated soils, but a minor component in agricultural soils. We also analyzed targeting genes trzN and atzABC with multiplex PCR, which indicated that trzN and atzABC combination included degraders were prevalent in Shandong agricultural and industrial soils. Due to elimination of enrichment bias, direct isolation was more favorable for the diversity analysis of atrazine degrading bacteria.

Key words: direct isolation, atrazinedegrading bacteria, trzN;atzA

CLC Number:

X172

LI Hongmei, LI Chengyun, LI Jishun, WEI Yanli, YANG Hetong, BAZHANAU Dzmitry. Direct detection and isolation of atrazinedegrading bacteria[J].SHANDONG SCIENCE, 2015, 28(6): 17-22.

References

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Direct detection and isolation of atrazinedegrading bacteria

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