直接分离于工业和农业土样中的阿特拉津降解菌的基因分型

doi:10.3976/j.issn.1002-4026.2015.06.005

Abstract

Abstract: We identified and classified 116 atrazinedegrading strains directly isolated from industrial and agricultural soil samples polluted by herbicides. We discovered 12 phylotypes among them through phylogenetic analysis of ERICPCR genotyping and 16S rRNA gene. Among the 12 phylotypes, we identified 8 phylotypes belonging to Arthrobacter bacteria in industrial soils, which are similar to phylogenetic groups of A.aurescens. We also detected identical genotypes within phylogenetic groups of Pseudomonas alcaliphila and Gulosibacter molinativorax in heavily contaminated no plant industrial soil. Only 3 phylotypes were distinguished in agricultural corn root soil. Bacteria genetically similar to Arthrobacter ureafaciens were ubiquitous and predominant atrazine degraders in agricultural soils. The other 2 phylotypes were distant Nocardioides spp.Genetic typing results indicate specific environment of genetic structures and diversity of atrazinedegrading microbial colonies. Contamination rate in industrial soils is the main factor affecting degrading diversity and genetic structures.

Key words: Atrazinedegrading bacteria, phylogenies, genetic typing, Rep-PCR

CLC Number:

S182

LI Chengyun，LI Hongmei，LI Jishun，YANG Hetong，BAZHANAU Dzmitry. Genetic typing of atrazinedegrading bacteria directly isolated from industrial and agricultural soil samples[J].SHANDONG SCIENCE, 2015, 28(6): 23-34.

References

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Genetic typing of atrazinedegrading bacteria directly isolated from industrial and agricultural soil samples

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