哈茨木霉LTR-2对蔬菜种子和幼苗耐盐性的影响及其作用机制

doi:10.3976/j.issn.1002-4026.2015.05.005

Abstract

Abstract: We addressed the impact of Trichoderma harzianum strain LTR-2 on seed germination, seedling growth and physiological and biochemical response of cucumber, radish and tomato under salinity stress. Results show that germination rate of vegetable seeds treated with LTR-2 is significantly higher than that of seeds untreated with LTR-2 under NaCl stress. Shoot length, fresh weight, chlorophyll content, net photosynthetic rate (Pn value) and antioxidant enzyme activity of vegetable seedlings treated with LTR-2 are significantly higher than those of seedlings without LTR-2 treatment. However, relative conductivity and MDA content are less than those without LTR-2 treatment. This indicates that T. harzianum strain LTR-2 can stimulate germination of vegetable seeds, growth and photosynthesis of seedlings, improve antioxidant capacity of seedlings, reduce membrane damage, and enhance salt tolerance of seedlings.

Key words: vegetable seeds, Trichoderma harzianum, salinity stress, salt tolerance

CLC Number:

S182

ZHAO Zhongjuan, HU Jindong, WU Xiaoqing, ZHAO Xiaoyan,LI Zhe, LI Jishun, YANG Hetong. Impact and mechanism of Trichoderma harzianum strain LTR-2 on salt tolerance of vegetable seeds and seedlings[J].SHANDONG SCIENCE, 2015, 28(5): 27-34.

References

［1］SZABOLES I Salinization of soil and water and its relation to desertification［J］ Desertification Control Bulletin, 1992, 21:32-37 ［2］李彬, 王志春, 孙志高, 等中国盐碱地资源与可持续利用研究［J］ 干旱地区农业研究, 2005, 23(20): 154-158 ［3］王燕, 赵哈林, 赵学勇,等 荒漠绿洲农田盐渍化过程中的水盐动态［J］ 水土保持学报, 2013, 27 (1): 186-192 ［4］HARMAN G E Multifunctional fungal plant symbionts: New tools to enhance plant growth and productivity［J］ New Phytol, 2011, 189(3): 647-649 ［5］HARMAN G E, HOWELL C R,VITERBO A,et alTrichoderma speciesopportunistic, avirulent plant symbionts ［J］ Nat Rev Microbiol, 2004, 2(1):43-56 ［6］BAE H, SICHER R C, KIM M S, et al The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao［J］ J Exp Bot, 2009, 60(11):3279-3295 ［7］MASTOURI F, BJRKMAN T, HARMAN G E Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings ［J］ Phytopathology, 2010, 100(11):1213-1221 ［8］SHORESH M, HARMAN G E ,MASTOURI F Induced systemic resistance and plant responses to fungal biocontrol agents ［J］ Annu Rev Phytopathol, 2010, 48:21-43 ［9］MASTOURI F, BJRKMAN T, HARMAN G E Trichoderma harzianum enhances antioxidant defense of tomato seedlings and resistance to water deficit ［J］ Molecular PlantMicrobe Interactions, 2012, 25(9):1264–1271 ［10］BROTMAN Y, LANDAU U, CUADROSINOSTROZA A , et alTrichodermaplant root colonization: Escaping early plant defense responses and activation of the antioxidant machinery for saline stress tolerance ［J］ PLoS Pathog, 2013, 9(3): 130-139 ［11］杨合同, 李纪顺, 陈凯,等 绿色木霉LTR2菌株及其制剂,中国：CN100340655 ［P］ 2007-10-03 ［12］李哲, 郭凯, 吴晓青,等 哈茨木霉LTR2对椒样薄荷耐盐生理特性的影响［J］ 山东科学, 2014,27(4): 17-24 ［13］张志良, 瞿伟菁，李小方 植物生理学实验指导［M］．3版北京：高等教育出版社, 2003 ［14］L S L, YANG A F, ZHANG K W, et al Increase of glycinebetaine synthesis improves drought tolerance in cotton［J］ Mol Breed, 2007, 20(3): 233-248 ［15］SEQUEIRA L, MINEO L Partial purification and kinetics of indoleacetic acid oxidase from tobacco roots［J］ Plant Physiol, 1966, 41（7）: 1200-1208 ［16］张宪政，谭桂茹,黄元极 植物生理学实验技术［M］ 沈阳: 辽宁科学技术出版社, 1989 ［17］高俊凤 植物生理学实验指导［M］ 北京: 高等教育出版社, 2006: 217-218 ［18］朱新广, 张其德．NaCl 对光合作用影响的研究进展［J］．植物学通报, 1999, 16(4): 332-338．［19］杨升, 张华新, 张丽 植物耐盐生理生化指标及耐盐植物筛选综述［J］ 西北林学院学报2010, 25(3):59 -65 ［20］CENTRITTO M, LORETO F, CHARTZOULAKIS K The use of low ［CO2］to estimate diffusional and nondiffusional limitations of photosynthetic capacity of saltstressed olive saplings ［J］ Plant Cell and Environment, 2003, 26(4): 585-594 ［21］QIU NW, LU Q T, LU CM Photosynthesis, photosystem II efficiency and the xanthophyll cycle in the saltadapted halophyte Atriplex centralasiatica ［J］ New Phytologist, 2003, 159(2): 479-486 ［22］McKAY H M, MASON W L Physiological in dicators of tolerance to cold storage in sitkspruce and douglas2fir seedlings ［J］Canadian Journal Forest Research, 1991, 21(6): 890-901 ［23］李合生 植物生理生化实验原理和技术［M］北京:高等教育出版社, 2000 ［24］王智明, 张峰举, 许兴 植物耐盐生理生化指标研究进展［J］ 湖北农业科学, 2014, 53(7): 1493-1496 ［25］ESTERBAUER H Cytotoxicity and genotoxicity of lipidoxidation products［J］ Am J Clin Nutr, 1993, 57（5）: 779S-786S ［26］WEINDLING RTrichoderma lignorum as a parasite of other soil fungi［J］ Phytopathology, 1932, 22:837-845 ［27］YILDIRIM E, TAYLOR A G, SPITTLER T D Ameliorative effects of biological treatments on growth of squash plants under salt stress ［J］ Sci Hortic, 2006, 111(1):1-6 ［28］BJORKMAN T, BLANCHARD L M, HARMAN G E Growth enhancement of shrunken2 (sh2) sweet corn by Trichoderma harzianum 129522: Effect of environmental stress［J］ Journal of Horticalture Science, 1998, 123:35-40 ［29］HARMAN G E Overview of mechanisms and uses of Trichoderma spp.［J］ Phytopathology, 2006, 96(2):190-194 ［30］HARMAN G E Myths and dogmas of biocontrol:Changes in perceptions derived from research on Trichoderma harzianum T22［J］ Plant Dis, 2000, 84(4):377-393

Metrics

Comments

Recommended 0

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits third parties to freely share (i.e., copy and redistribute the material in any medium or format) and adapt (i.e., remix, transform, or build upon the material) the articles published in this journal, provided that appropriate credit is given, a link to the license is provided, and any changes made are indicated. The material may not be used for commercial purposes. For details of the CC BY-NC 4.0 license, please visit: https://creativecommons.org/licenses/by-nc/4.0

[1]	YANG Qian,YANG Bao-shan,QIN Guang-hua,WANG Hui,SHI Jia-xing,LIU Xue-ting. Salt tolerance evaluation and selection of salt tolerance indexes for new varieties of willow seedlings [J]. Shandong Science, 2022, 35(3): 107-114.
[2]	WANG Yi-lian, ZHAO Zhong-juan, YANG Kai, LU Chang-hou, YU Yi, XU Wei-sheng, LI Ji-shun. Effects of Trichoderma harzianum T11-W and apple branch sawdust on cucumber seedling growth [J]. Shandong Science, 2021, 34(3): 36-41.
[3]	LIU Bao-jun, WANG Yi-lian, HU Jin-dong, LI Ji-shun, YANG He-tong. Biocontrol effect evaluation of combined Trichoderma harzianum and Bacillus cereus against root-knot nematode Meloidogyne spp. on tomatoes [J]. Shandong Science, 2019, 32(5): 71-75.
[4]	WANG Yi-lian，LI Ji-shun, YANG He-tong. Identification of Trichoderma T11W and its parasitism to Meloidogyne incognita eggs [J]. SHANDONG SCIENCE, 2014, 27(6): 38-42.
[5]	LI Zhe, GUO Kai, WU Xiao-Qing, CHEN Quan, LI Ji-Shun, YANG Ge-Tong. Impacts of Trichoderma harzianum LTR-2 on salttolerant physiological characteristics of peppermint (Mentha piperita L.) [J]. SHANDONG SCIENCE, 2014, 27(4): 17-24.

Impact and mechanism of Trichoderma harzianum strain LTR-2 on salt tolerance of vegetable seeds and seedlings

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0