一株解蛋白芽孢杆菌的分离及生理特性研究

doi:10.3976/j.issn.1002-4026.2025065

Abstract

Abstract: Abacterial strain capable of producing exopolysaccharides (EPS) was isolated from soil and identified asBacillus proteolyticusstrainbased on morphological characteristics and 16S rDNA sequence analysis. The strain was designated as LY0. Experimental results showed that LY0 secreted various extracellular products, including extracellular enzymes and EPS. Antimicrobial activity, assessed by inhibition zone assays, showed that LY0 inhibited the growth of several bacteria such asEscherichia coliandStaphylococcus aureus. Viscosity measurements of LY0 liquid cultures revealed a peak at 24 h. Different carbon sources were added to the fermentation medium, and EPS yield was determined using the phenol–sulfuric acid method following purification with dialysis and trichloroacetic acid. The results showed that, compared with other carbon sources, sucrose yielded the highest EPS production (32.2 mg/L), suggesting that sucrose is the optimalcarbon source for EPS production by LY0. TheBacillus proteolyticusstrain obtained in this study can be used for microbial EPS production and holdsbroad application prospects.

Key words: Bacillus proteolyticus, antibacterial activity, extracellular enzymes, exopolysaccharides, viscosity, fermentation condition optimization

CLC Number:

Q939.1

ZHENG Keran, TAN Fumin, LI Qifeng, WANG Tong, ZHANG Xiaomei, GAO Cuijuan. Isolation and physiological characterization of a bacillus proteolyticus strain[J].Shandong Science, 0, (): 1-.

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Isolation and physiological characterization of a bacillus proteolyticus strain

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