不同粒径PS-MNPs对铜绿微囊藻的毒理效应

doi:10.3976/j.issn.1002-4026.20240076

Abstract

Abstract:

Micro/nanoplastics (MNPs) and microalgae are widely distributed in water and MNPs that adhere to the surface of microalgae or enter their internal structures will enter the food chain in large quantities, posing a great threat to aquatic ecosystems. The physiological effects of MNPs on algae vary depending on their particle sizes. In this study, polystyrene micro/nanoplastics(PS-MNPs) particles were selected as target pollutants to investigate their toxicological effects on Microcystis aeruginosa (FACHB 905) at different concentrations (5, 10, 50 and 250 mg/L) and particle sizes (100 μm and 80 nm). Results showed that the inhibition effect of 80 nm PS-MNPs exerted a more potent inhibitory effect on the growth of algal, chlorophyll a and phycobiliprotein synthesis than 100 μm PS-MNPs, and the inhibitory effect was more obvious with the increase of PS concentration. In addition, the activities of catalase (CAT), malondialdehyde dehydrogenase (MDA) and glutathione (GSH) in microalgae cells were significantly increased under the stress of high concentration of PS-MNPs, indicating that high concentration of PS-MNPs caused oxidative damage to algal cells, and smaller PS-MNPs particles can lead to more severe oxidative damage. The toxicity of PS-MNPs with different particle sizes toward M. aeruviosa mainly led to cell destruction through surface adsorption, which affected photosynthesis and energy metabolism of algal cells, hindering normal physiological and biochemical reactions in algal cells. This study, by exploring the toxicity mechanism of PS-MNPs to microcystis aeruginosa, is of great significance for the risk assessment of PS-MNPs, and provides a theoretical basis for the prevention of M. aeruginosa bloom.

Key words: micro/nanoplastics, polystyrene, Microcystis aeruginosa, toxicological effects

CLC Number:

YE Hongyan, ZHAO Zihan, LIU Chunhui, YAO Yihan, YUE Shizhong, WANG Ruiping. Toxicological effects of micro/nanoplastics with different particle sizes on Microcystis aeruginosa[J].Shandong Science, 2025, 38(4): 95-105.

Figures/Tables 6

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References 43

[1]	Plastic Europe. Plastics:the fast faces 2023[EB/OL].[2024-05-23]. https://plasticseurope.org/knowledge-hub/plastics-the-fast-facts-2023/.
[2]	ALIMI O S, CLAVEAU-MALLET D, KURUSU R S, et al. Weathering pathways and protocols for environmentally relevant microplastics and nanoplastics: what are we missing?[J]. Journal of Hazardous Materials, 2022, 423(Pt A): 126955. DOI: 10.1016/j.jhazmat.2021.126955.
[3]	GEYER R, JAMBECK J R, LAW K L. Production, use, and fate of all plastics ever made[J]. Science Advances, 2017, 3(7): e1700782. DOI: 10.1126/sciadv.1700782.
[4]	LAW K L, THOMPSON R C. Microplastics in the seas: concern is rising about widespread contamination of the marine environment by microplastics[J]. Science, 2014, 345 (6193):144-145.
[5]	HANIF M A, IBRAHIM N, DAHALAN F A, et al. Microplastics and nanoplastics: recent literature studies and patents on their removal from aqueous environment[J]. The Science of the Total Environment, 2022, 810: 152115. DOI: 10.1016/j.scitotenv.2021.152115.
[6]	FERREIRA I, VENÂNCIO C, LOPES I, et al. Nanoplastics and marine organisms: what has been studied?[J]. Environmental Toxicology and Pharmacology, 2019, 67: 1-7. DOI: 10.1016/j.etap.2019.01.006. pmid: 30685594
[7]	SINGH S, KUMAR NAIK T S S, ANIL A G, et al. Micro (nano) plastics in wastewater: a critical review on toxicity risk assessment, behaviour, environmental impact and challenges[J]. Chemosphere, 2022, 290: 133169. DOI: 10.1016/j.chemosphere.2021.133169.
[8]	ZHOU Y F, GUI L, WEI W B, et al. Low particle concentrations of nanoplastics impair the gut health of medaka[J]. Aquatic Toxicology, 2023, 256: 106422. DOI: 10.1016/j.aquatox.2023.106422.
[9]	SCHRÖTER L, VENTURA N. Nanoplastic toxicity: insights and challenges from experimental model systems[J]. Small, 2022, 18(31): e2201680. DOI: 10.1002/smll.202201680.
[10]	SHEN M C, ZHANG Y X, ZHU Y, et al. Recent advances in toxicological research of nanoplastics in the environment: a review[J]. Environmental Pollution, 2019, 252(Pt A): 511-521. DOI: 10.1016/j.envpol.2019.05.102. pmid: 31167159
[11]	BOUWMEESTER H, HOLLMANP C H, PETERS R J B. Potential health impact of environmentally released micro- and nanoplastics in the human food production chain: experiences from nanotoxicology[J]. Environmental Science & Technology, 2015, 49(15): 8932-8947. DOI: 10.1021/acs.est.5b01090.
[12]	WANG L W, WU W M, BOLAN N S, et al. Environmental fate, toxicity and risk management strategies of nanoplastics in the environment: current status and future perspectives[J]. Journal of Hazardous Materials, 2021, 401: 123415. DOI: 10.1016/j.jhazmat.2020.123415.
[13]	ZHENG X W, ZHANG W Z, YUAN Y, et al. Growth inhibition, toxin production and oxidative stress caused by three microplastics in Microcystis aeruginosa[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111575. DOI: 10.1016/j.ecoenv.2020.111575.
[14]	干牧凡, 张妍, 时鹏, 等. 水生生态系统中微塑料对微藻的生态毒理效应研究进展[J]. 生态毒理学报, 2023, 18(1): 217-231. DOI: 10.7524/AJE.1673-5897.20220215001.
[15]	CHAE Y, KIM D, KIM S W, et al. Trophic transfer and individual impact of nano-sized polystyrene in a four-species freshwater food chain[J]. Scientific Reports, 2018, 8(1): 284. DOI: 10.1038/s41598-017-18849-y.
[16]	MATTSSON K, JOHNSON E V, MALMENDAL A, et al. Brain damage and behavioural disorders in fish induced by plastic nanoparticles delivered through the food chain[J]. Scientific Reports, 2017, 7(1): 11452. DOI: 10.1038/s41598-017-10813-0.
[17]	GOMES T, ALMEIDA A C, GEORGANTZOPOULOU A. Characterization of cell responses in Rhodomonas baltica exposed to PMMA nanoplastics[J]. The Science of the Total Environment, 2020, 726: 138547. DOI: 10.1016/j.scitotenv.2020.138547.
[18]	HANACHI P, KHOSHNAMVAND M, WALKER T R, et al. Nano-sized polystyrene plastics toxicity to microalgae Chlorella vulgaris: toxicity mitigation using humic acid[J]. Aquatic Toxicology, 2022, 245: 106123. DOI: 10.1016/j.aquatox.2022.106123.
[19]	BERGAMI E, PUGNALINI S, VANNUCCINI M L, et al. Long-term toxicity of surface-charged polystyrene nanoplastics to marine planktonic species Dunaliella tertiolecta and Artemia franciscana[J]. Aquatic Toxicology, 2017, 189: 159-169. DOI: 10.1016/j.aquatox.2017.06.008.
[20]	FENG L J, SUN X D, ZHU F P, et al. Nanoplastics promote microcystin synthesis and release from cyanobacterial Microcystis aeruginosa[J]. Environmental Science & Technology, 2020, 54(6): 3386-3394. DOI: 10.1021/acs.est.9b06085.
[21]	SEOANE M, GONZÁLEZ-FERNÁNDEZ C, SOUDANT P, et al. Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile[J]. Environmental Pollution, 2019, 251: 363-371. DOI: 10.1016/j.envpol.2019.04.142.
[22]	GAO G, ZHAO X, JIN P, et al. Current understanding and challenges for aquatic primary producers in a world with rising micro- and nano-plastic levels[J]. Journal of Hazardous Materials, 2021, 406: 124685. DOI: 10.1016/j.jhazmat.2020.124685.
[23]	GONZÁLEZ-FERNÁNDEZ C, LE GRAND F, BIDEAU A, et al. Nanoplastics exposure modulate lipid and pigment compositions in diatoms[J]. Environmental Pollution, 2020, 262: 114274. DOI: 10.1016/j.envpol.2020.114274.
[24]	SJOLLEMA S B, REDONDO-HASSELERHARM P, LESLIE H A, et al. Do plastic particles affect microalgal photosynthesis and growth?[J]. Aquatic Toxicology, 2016, 170: 259-261. DOI: 10.1016/j.aquatox.2015.12.002. pmid: 26675372
[25]	MAO Y F, AI H N, CHEN Y, et al. Phytoplankton response to polystyrene microplastics: perspective from an entire growth period[J]. Chemosphere, 2018, 208: 59-68. DOI: 10.1016/j.chemosphere.2018.05.170. pmid: 29860145
[26]	YE S S, RAO M Y, XIAO W Y, et al. The relative size of microalgal cells and microplastics determines the toxicity of microplastics to microalgae[J]. Process Safety and Environmental Protection, 2023, 169: 860-868. DOI: 10.1016/j.psep.2022.11.077.
[27]	LIU G, JIANG R F, YOU J, et al. Microplastic impacts on microalgae growth: effects of size and humic acid[J]. Environmental Science & Technology, 2020, 54(3): 1782-1789. DOI: 10.1021/acs.est.9b06187.
[28]	ZHOU J Y, GAO L, LIN Y Y, et al. Micrometer scale polystyrene plastics of varying concentrations and particle sizes inhibit growth and upregulate microcystin-related gene expression in Microcystis aeruginosa[J]. Journal of Hazardous Materials, 2021, 420: 126591. DOI: 10.1016/j.jhazmat.2021.126591.
[29]	孙炎, 刘千龙, 罗肇河, 等. 聚苯乙烯微塑料对东海原甲藻生长的影响[J]. 应用海洋学学报, 2021, 40(4): 636-642. DOI: 10.3969/J.ISSN.2095-4972.2021.04.010.
[30]	YI X L, CHI T T, LI Z C, et al. Combined effect of polystyrene plastics and triphenyltin chloride on the green algae Chlorella pyrenoidosa[J]. Environmental Science and Pollution Research International, 2019, 26(15): 15011-15018. DOI: 10.1007/s11356-019-04865-0.
[31]	LUO H W, XIANG Y H, HE D Q, et al. Leaching behavior of fluorescent additives from microplastics and the toxicity of leachate to Chlorella vulgaris[J]. The Science of the Total Environment, 2019, 678: 1-9. DOI: 10.1016/j.scitotenv.2019.04.401.
[32]	LIN S J, BHATTACHARYA P, RAJAPAKSE N C, et al. Effects of quantum dots adsorption on algal photosynthesis[J]. The Journal of Physical Chemistry C, 2009, 113(25): 10962-10966. DOI: 10.1021/jp904343s.
[33]	TUNALI M, UZOEFUNA E N, TUNALI M M, et al. Effect of microplastics and microplastic-metal combinations on growth and chlorophyll a concentration of Chlorella vulgaris[J]. The Science of the Total Environment, 2020, 743: 140479. DOI: 10.1016/j.scitotenv.2020.140479.
[34]	WU D, WANG T, WANG J, et al. Size-dependent toxic effects of polystyrene microplastic exposure on Microcystis aeruginosa growth and microcystin production[J]. The Science of the Total Environment, 2021, 761: 143265. DOI: 10.1016/j.scitotenv.2020.143265.
[35]	XIAO Y, JIANG X F, LIAO Y C, et al. Adverse physiological and molecular level effects of polystyrene microplastics on freshwater microalgae[J]. Chemosphere, 2020, 255: 126914. DOI: 10.1016/j.chemosphere.2020.126914.
[36]	YAN Z, XU L M, ZHANG W M, et al. Comparative toxic effects of microplastics and nanoplastics on Chlamydomonas reinhardtii: growth inhibition, oxidative stress, and cell morphology[J]. Journal of Water Process Engineering, 2021, 43: 102291. DOI: 10.1016/j.jwpe.2021.102291.
[37]	WU Y M, GUO P Y, ZHANG X Y, et al. Effect of microplastics exposure on the photosynthesis system of freshwater algae[J]. Journal of Hazardous Materials, 2019, 374: 219-227. DOI: 10.1016/j.jhazmat.2019.04.039. pmid: 31005054
[38]	MAHANA A, GULIY O I, MEHTA S K. Accumulation and cellular toxicity of engineered metallic nanoparticle in freshwater microalgae: current status and future challenges[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111662. DOI: 10.1016/j.ecoenv.2020.111662.
[39]	NIE J H, SHEN Y, ROSHDY M, et al. Polystyrene nanoplastics exposure caused defective neural tube morphogenesis through caveolae-mediated endocytosis and faulty apoptosis[J]. Nanotoxicology, 2021, 15(7): 885-904. DOI: 10.1080/17435390.2021.1930228.
[40]	LIN J Q, ALEXANDER-KATZ A. Cell membranes open “doors” for cationic nanoparticles/biomolecules: insights into uptake kinetics[J]. ACS Nano, 2013, 7(12): 10799-10808. DOI: 10.1021/nn4040553.
[41]	VAN LEHN R C, ALEXANDER-KATZ A. Penetration of lipid bilayers by nanoparticles with environmentally-responsive surfaces: simulations and theory[J]. Soft Matter, 2011, 7(24): 11392-11404. DOI: 10.1039/C1SM06405C.
[42]	王素春, 刘光洲, 张欢, 等. 微塑料对微藻的毒性效应研究进展[J]. 海洋环境科学, 2019, 38(2): 192-197.DOI: 10.13634/j.cnki.mes.2019.02.005.
[43]	BHATTACHARYA P, LIN S J, TURNER J P, et al. Physical adsorption of charged plastic nanoparticles affects algal photosynthesis[J]. The Journal of Physical Chemistry C, 2010, 114(39): 16556-16561. DOI: 10.1021/jp1054759.

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Toxicological effects of micro/nanoplastics with different particle sizes on Microcystis aeruginosa

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