不同体积分数二甲基亚砜对DNA变性的影响

doi:10.3976/j.issn.1002-4026.2022.03.016

Abstract

Abstract:

The effects of different volume fractions of dimethyl sulfoxide (DMSO) on plasmid and linear DNA denaturation were investigated using atomic force microscopy and dynamic light scattering. It was found that a low volume fraction of 1% DMSO solution can also induce local DNA denaturation, which can be directly observed by atomic force microscopy. Simultaneously, due to the limitations of plasmid DNA linking number and single-stranded DNA generation, atomic force microscopy and dynamic light scattering showed that DNA gradually forms a superhelix structure with an increase in the volume fraction of DMSO. The average particle size of pBR322 DNA, 5 000 base pair DNA, and λ-DNA decreased from about (474±10) nm, (554±11) nm, and (871±17) nm, respectively, in 0 DMSO solution to (257±8) nm, (282±18) nm, and (449±21) nm, respectively, in 10% DMSO solution.

Key words: :DNA, denaturation, DNA topology structure, organic denaturation reagent, particle size

CLC Number:

XU Min,WANG Yan-wei,YANG Guang-can. Effects of different volume fractions of dimethyl sulfoxide on DNA denaturation[J].Shandong Science, 2022, 35(3): 131-137.

Figures/Tables 9

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References 24

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Effects of different volume fractions of dimethyl sulfoxide on DNA denaturation

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