Abstract: In this study, we developed a method based on liquid chromatography-mass spectrometry to detect content changes in animals administered commercially available tamsulosin hydrochloride sustained-release tablets. Beagle dogs were used as the experimental animals to construct drug pharmacokinetic curves as well as to verify the reliability of the method. The column used was the Zorbax Extend-C18 column (150 mm×4.6 mm, 5 μm). C18 (4.0 mm×3.0 mm) was used as the guard column, and the mobile phase was methanol-water-formic acid (V（methanol）:V(water):V(formic)=85:14:1). The flow rate was 0.6 mL/min, column temperature was 30 ℃, and injection volume was 20 μL. The ESI ion source positive ion multiple reaction monitoring mode was used to select ion pairs 409.3→228.2 and 256.3→167.1 for determination. The results showed that tamsulosin hydrochloride has a good linear correlation in the concentration range of 0.1~10.0 ng/mL. The precision was 7.1%, and the recovery rate of extraction was 92%. The content changes of tamsulosin hydrochloride sustained-release tablets in Beagle dogs were accurately described. The above method has high sensitivity, good specificity, and high precision and can quickly and easily detect the content changes in animals administered commercially available tamsulosin hydrochloride sustained-release tablets.

Key words: tamsulosin hydrochloride sustained-release tablets')">

tamsulosin hydrochloride sustained-release tablets, liquid chromatography-mass spectrometry, Beagle dog, body content; pharmacokinetics