多糖,铁皮石斛,">提取,">脱蛋白, 多糖,铁皮石斛,">提取,">脱蛋白, Dendrobium officinale,extraction,"/> 铁皮石斛原球茎多糖提取及脱蛋白工艺研究

山东科学 ›› 2016, Vol. 29 ›› Issue (3): 7-12.doi: 10.3976/j.issn.1002-4026.2016.03.002

• 中药与天然活性产物 • 上一篇    下一篇

铁皮石斛原球茎多糖提取及脱蛋白工艺研究

张英洁1,2，耿岩玲２，王晓１,２，刘峰2*   

  1. 1. 山东师范大学生命科学学院 , 山 东 济 南 250014; 2. 山 东 省 分 析 测 试 中 心 ,山东省中药质量控制技术重点实验室,山东济南250014
  • 收稿日期:2016-03-22 出版日期:2016-06-20 发布日期:2016-06-20
  • 通信作者: 刘 峰( 1981 - ),男,博士,助理研究员,研究方向为天然产物研究与开发 E-mail:liufeng8109@163. com
  • 作者简介:张英洁(1991-),女,硕士研究生,研究方向为天然产物分离纯化?Email: 1009171097@ qq. com
  • 基金资助:
    国家高技术研究发展计划(863计划)(2014AA02220105);山东省自然科学基金(ZR2014YL005)

Extraction and deproteinization technology for polysaccharide from Dendrobium officinale protocorm

ZHANG Ying-jie1,2, GEＮＧ Ｙan-ling2, WANG Xiao1,2, LIU Feng2*   

  1.  
    1. School of Life Sciences, Shandong Normal University, Jinan 250014, China; 2. Shandong Provincial Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Shandong Analysis and
    Test Center, Jinan 250014, China
  • Received:2016-03-22 Online:2016-06-20 Published:2016-06-20

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摘要：

      研究了铁皮石斛原球茎培养物中多糖提取工艺并进行了不同脱蛋白方法的比较?以提取温度?提取时间和料液比三因素进行正交实验,得到多糖最佳提取工艺为提取温度90℃?提取时间60 min?料液比1∶40(g/mL),多糖提取率78.51%?通过一系列单因素实验和正交优化实验,分析比较Sevage法?盐酸法?三氯乙酸法和木瓜蛋白酶法脱蛋白工艺?结果显示,Sevage法第5次洗脱效果最好,多糖质量分数43.81%,蛋白质质量分数9.67%;盐酸在体积分数25%时效果最好,多糖质量分数52.17%,蛋白质质量分数为4.82%;三氯乙酸质量浓度为0.03 g/mL时,多糖质量分数最高35.50%,蛋白质质量分数7.34%;木瓜蛋白酶法反应时间90 min?温度70℃?酶含量30U时,多糖质量分数85.01%,蛋白质质量分数为5.89%,效果最为理想?

关键词: 多糖">多糖')">">, 铁皮石斛">铁皮石斛')">">, 提取">提取')">">, 脱蛋白')">">脱蛋白, We addressed polysaccharide extraction technology from Dendrobium officinale protocorm and compared different deproteinization methods. We conducted orthogonal experiments with extraction temperature and time, and the ratio of solid and liquid as parameters. We found its optimum extraction conditions included extraction temperature of 90 ℃, extraction time of 60 minutes and solid/
liquid ratio of 1∶40 ( g/ mL) , and extraction efficiency could reach 78.51 %. We further compared Sevage, hydrochloricacid ( HCl) ,
trichloroacetic acid ( TCA ) and papain deproteinization technologies through a series of single element experiments and orthogonal optimization experiments. Experimental results show that: the fifth elution effect of Sevage is optimal,polysaccharide content of 43. 81% and protein content of 9. 67%. Elution effect is optimal, polysaccharide content of 52. 17% and protein content of 4. 82%, when HCL concentration is 25%. When TCA concentration is 0. 03 g/ mL, polysaccharide content is 35. 50 % and protein content is 7. 34 %. For papain
method, optimum extraction conditions are enzymolysis time of 90 min, temperature of 70 ℃, and enzyme content of 30 U. The corresponding
best effect is polysaccharide content of 85.01% and protein content of 5.89%.

Key words: polysaccharide, deproteinization, Dendrobium officinale')">Dendrobium officinale, extraction

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  • 引用本文

    张英洁，耿岩玲，王晓，刘峰. 铁皮石斛原球茎多糖提取及脱蛋白工艺研究[J]. 山东科学, 2016, 29(3): 7-12.

    ZHANG Ying-jie, GEＮＧ Ｙan-ling, WANG Xiao, LIU Feng. Extraction and deproteinization technology for polysaccharide from Dendrobium officinale protocorm[J]. SHANDONG SCIENCE, 2016, 29(3): 7-12.

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