一株菲降解菌的分离、鉴定及最佳培养条件的优化

doi:10.3976/j.issn.1002-4026.2015.02.013

山东科学 ›› 2015, Vol. 28 ›› Issue (2): 75-80.doi: 10.3976/j.issn.1002-4026.2015.02.013

一株菲降解菌的分离、鉴定及最佳培养条件的优化

黄玉杰，邱维忠，高永超，孔学，张闻，王加宁

山东省科学院生物技术研究中心，山东省应用微生物重点实验室，山东济南 250014

收稿日期:2014-07-23 出版日期:2015-04-20 发布日期:2015-04-20
作者简介:黄玉杰（1977-），女，副研究员，研究方向为污染土壤生物修复。
基金资助:
山东省科学院先导科技专项（2013）；国家高技术研究发展计划（863计划）（2013AA06A210）；山东省科学技术发展计划（2014GSF117019）

Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria

HUANG Yujie, QIU Weizhong, GAO Yongchao, KONG Xue, ZHANG Wen, WANG Jianing

Shandong Provincial Key laboratory of Applied Microbiology, Biotechnology Center, Shandong Academy of Sciences, Jinan 250014,China

Received:2014-07-23 Online:2015-04-20 Published:2015-04-20

摘要/Abstract

摘要： 从石油污染场地分离到一株可降解菲的菌株SP3，经生理生化鉴定和16S rDNA序列对比分析，初步鉴定该菌株为分枝杆菌（Mycobacterium sp.），该菌能够以菲为唯一碳源，在菲浓度为100 μg/mL的无机盐培养基中，28 ℃摇床培养7 d降解率达到了99%以上。对该菌进行了最佳发酵培养基及培养条件的优化研究，其最佳培养基配方为淀粉1%、牛肉膏1%、Na2HPO4 0.2%、NaH2PO4 0.2%，并通过单因素实验法确定了最佳培养条件为温度30 ℃，初始pH =7.5，培养时间12 h。

关键词: 菲, 多环芳烃, 降解, 培养基优化

Abstract: A phenanthrene degradable strain SP3 was isolated from petrolcontaminated soil. It was roughly identified as Mycobacterium sp.through physiological and biochemical identification and contrast analysis of 16S rDNA sequences. Phenanthrene could be served as its unique carbon source.The degradation rate is more than 99% in such conditions as mineral salt medium of phenanthrene concentration of 100 μg/mL,shaking culture of 28 ℃ and 7 days. We discovered that optimal culture medium of strain SP3 included starch of 1%, beef extract of 1%,Na2HPO4 of 0.2%and NaH2PO4 of 0.2%. We determined its optimum culture conditions of temperature of 30 ℃, initial pH of 7.5 and culture time of 12 h through single factor experiment.

Key words: optimization of medium, degradation, phenanthrene, PAHs

中图分类号:

黄玉杰，邱维忠，高永超，孔学，张闻，王加宁. 一株菲降解菌的分离、鉴定及最佳培养条件的优化[J]. 山东科学, 2015, 28(2): 75-80.

HUANG Yujie, QIU Weizhong, GAO Yongchao, KONG Xue, ZHANG Wen, WANG Jianing. Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria[J]. SHANDONG SCIENCE, 2015, 28(2): 75-80.

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一株菲降解菌的分离、鉴定及最佳培养条件的优化

Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria

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