阿特拉津降解细菌的直接检测及分离

doi:10.3976/j.issn.1002-4026.2015.06.004

山东科学 ›› 2015, Vol. 28 ›› Issue (6): 17-22.doi: 10.3976/j.issn.1002-4026.2015.06.004

阿特拉津降解细菌的直接检测及分离

李红梅, 李成云, 李纪顺, 魏艳丽, 杨合同, BAZHANAU Dzmitry

山东省科学院生物研究所，山东省应用微生物重点实验室，山东济南 250014

收稿日期:2015-07-30 出版日期:2015-12-20 上线日期:2015-12-20
作者简介:李红梅（1977-），女，助理研究员，硕士研究生，研究方向为应用微生物。
基金资助:
外专千人计划（WQ20123700079）；泰山学者岗位项目（鲁证办字［2012］109号）；山东省农业重大应用技术创新项目；山东省优秀青年基金（BS2014SW030）；山东省科学院青年基金（2014QN021）

Direct detection and isolation of atrazinedegrading bacteria

LI Hongmei, LI Chengyun, LI Jishun, WEI Yanli, YANG Hetong, BAZHANAU Dzmitry

Shandong Provincial Key Laboratory of Applied Microbiology,Biology Institute,Shandong Academy of Sciences, Jinan 250014, China

Received:2015-07-30 Published:2015-12-20 Online:2015-12-20

摘要/Abstract

摘要： 摘要：利用改良的 SM固体培养基，对受除草剂污染的土壤中阿特拉津降解菌进行了检测、计数及分离。研究发现，在工业污染土壤中，阿特拉津降解菌群成为微生物群落的主要组成部分，而在农业土壤中仅占次要地位。用多重PCR分析了靶向基因trzN 和 atzABC，表明含trzN-atzBC基因组合的降解菌在山东省工业及农业土壤中占主导地位。因为富集偏差的消除，直接分离方法更利于阿特拉津降解菌多样性分析的研究。

关键词: 直接分离, trzN, atzABC, 阿特拉津降解菌

Abstract: We detected, enumerated and isolated atrazinedegrading bacteria from herbicide contaminated soil by improved agar SM. We found that atrazine degraders constituted a major component of microbial communities in industrially contaminated soils, but a minor component in agricultural soils. We also analyzed targeting genes trzN and atzABC with multiplex PCR, which indicated that trzN and atzABC combination included degraders were prevalent in Shandong agricultural and industrial soils. Due to elimination of enrichment bias, direct isolation was more favorable for the diversity analysis of atrazine degrading bacteria.

Key words: direct isolation, atrazinedegrading bacteria, trzN;atzA

中图分类号:

X172

李红梅, 李成云, 李纪顺, 魏艳丽, 杨合同, BAZHANAU Dzmitry. 阿特拉津降解细菌的直接检测及分离[J]. 山东科学, 2015, 28(6): 17-22.

LI Hongmei, LI Chengyun, LI Jishun, WEI Yanli, YANG Hetong, BAZHANAU Dzmitry. Direct detection and isolation of atrazinedegrading bacteria[J]. SHANDONG SCIENCE, 2015, 28(6): 17-22.

参考文献

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阿特拉津降解细菌的直接检测及分离

Direct detection and isolation of atrazinedegrading bacteria

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