直接分离于工业和农业土样中的阿特拉津降解菌的基因分型

doi:10.3976/j.issn.1002-4026.2015.06.005

山东科学 ›› 2015, Vol. 28 ›› Issue (6): 23-34.doi: 10.3976/j.issn.1002-4026.2015.06.005

直接分离于工业和农业土样中的阿特拉津降解菌的基因分型

李成云，李红梅，李纪顺，杨合同，BAZHANAU Dzmitry

山东省科学院中日友好生物技术研究中心，山东济南 250014

收稿日期:2015-07-24 出版日期:2015-12-20 发布日期:2015-12-20
作者简介:李成云( 1985 － ) ，女，博士，研究方向为植物与细菌间互作、定殖。
基金资助:
外专千人计划（WQ20123700079）；泰山学者岗位项目（鲁证办字［2012］109号）；山东省农业重大应用技术创新项目；山东省优秀青年基金（BS2014SW030）；山东省科学院青年基金（2014QN021）

Genetic typing of atrazinedegrading bacteria directly isolated from industrial and agricultural soil samples

LI Chengyun，LI Hongmei，LI Jishun，YANG Hetong，BAZHANAU Dzmitry

Biotechnology Center, Shandong Academy of Sciences,Jinan 250014,China

Received:2015-07-24 Online:2015-12-20 Published:2015-12-20

摘要/Abstract

摘要： 对农药污染的工业和农业土样中获得的116株阿特拉津降解菌进行分类鉴定，降解菌通过ERICPCR和16S rRNA系统进化树分析发现了12种种系型。其中工业土样中鉴定出隶属于节杆菌属的8种种系型降解菌，具有与金黄节杆菌系统进化群体类似的基因种。从无植物生长的工业重度污染土样中检测到嗜碱假单胞菌和Gulosibacter molinativorax系统进化群体间存在着同一种基因型。农业玉米根际土样只分离到3种种系型，与产脲节杆菌类似的基因型是玉米根际普遍存在并占主导地位的降解菌，另外2种种系型代表着具有环境特异性的两个不同起源的类诺卡氏菌分支。基因分型结果暗示着阿特拉津降解群体的多样性和遗传结构的环境特异性，工业土壤中的污染率是影响降解群体多样性和遗传结构的主要因素。

关键词: 基因组重复序列（Rep）-PCR, 阿特拉津降解菌, 进化树, 基因分型

Abstract: We identified and classified 116 atrazinedegrading strains directly isolated from industrial and agricultural soil samples polluted by herbicides. We discovered 12 phylotypes among them through phylogenetic analysis of ERICPCR genotyping and 16S rRNA gene. Among the 12 phylotypes, we identified 8 phylotypes belonging to Arthrobacter bacteria in industrial soils, which are similar to phylogenetic groups of A.aurescens. We also detected identical genotypes within phylogenetic groups of Pseudomonas alcaliphila and Gulosibacter molinativorax in heavily contaminated no plant industrial soil. Only 3 phylotypes were distinguished in agricultural corn root soil. Bacteria genetically similar to Arthrobacter ureafaciens were ubiquitous and predominant atrazine degraders in agricultural soils. The other 2 phylotypes were distant Nocardioides spp.Genetic typing results indicate specific environment of genetic structures and diversity of atrazinedegrading microbial colonies. Contamination rate in industrial soils is the main factor affecting degrading diversity and genetic structures.

Key words: Atrazinedegrading bacteria, phylogenies, genetic typing, Rep-PCR

中图分类号:

S182

李成云，李红梅，李纪顺，杨合同，BAZHANAU Dzmitry. 直接分离于工业和农业土样中的阿特拉津降解菌的基因分型[J]. 山东科学, 2015, 28(6): 23-34.

LI Chengyun，LI Hongmei，LI Jishun，YANG Hetong，BAZHANAU Dzmitry. Genetic typing of atrazinedegrading bacteria directly isolated from industrial and agricultural soil samples[J]. SHANDONG SCIENCE, 2015, 28(6): 23-34.

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直接分离于工业和农业土样中的阿特拉津降解菌的基因分型

Genetic typing of atrazinedegrading bacteria directly isolated from industrial and agricultural soil samples

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