竹红菌素中试提纯工艺及抑菌活性研究

doi:10.3976/j.issn.1002-4026.2023.02.002

Abstract

Abstract:

To study the pilot-scale purification process and bacteriostatic effect of hypocrellin during the pilot-scale production, we extracted hypocrellin using different solvents and extraction methods. Then, we investigated the purification effects of the hypocrellin using salting-out, physical cooling, and ultrafiltration methods. The results showed that the amount of hypocrellin extracted using the jet pulverization method could reach up to 21.4 mg/g, and its density could exceed 90% after its purification via ultrafiltration. The analysis of the bacteriostatic activity of hypocrellin showed that it had good thermal stability and inhibitory effects against Escherichia coli and Candida albicans. We found that hypocrellin has natural antibacterial properties and good thermal stability, is easy to extract and purify, and can be used as a bacteriostatic drug in medicine and other applications.

Key words: hypocrellin, extraction, purification, stability, bacteriostatic activity

CLC Number:

R284.2

SUN Botong, LI Hongliang, HAN Rubing, WANG Ying, ZHANG Sichen. Study on pilot-scale purification process and antibacterial activity of hypocrellin[J].Shandong Science, 2023, 36(2): 8-15.

Figures/Tables 6

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Table 1

References 26

[1]	林海萍, 陈声明, 陈超龙. 一种值得开发利用的药用真菌:竹黄[J]. 浙江林业科技, 2002, 22(1): 77-80. DOI:10.3969/j.issn.1001-3776.2002.01.023. doi: 10.3969/j.issn.1001-3776.2002.01.023
[2]	MORAKOTKARN D, KAWASAKI H, SEKI T. Molecular diversity of bamboo-associated fungi isolated from Japan[J]. FEMS Microbiology Letters, 2007, 266(1): 10-19. DOI:10.1111/j.1574-6968.2006.00489.x. doi: 10.1111/j.1574-6968.2006.00489.x pmid: 17092298
[3]	钱瑞, 任锡毅, 刘永翔, 等. 基于广泛靶向代谢组学的竹黄活性成分分析[J]. 菌物学报, 2019, 38(5): 631-645. DOI:10.13346/j.mycosystema.180321. doi: 10.13346/j.mycosystema.180321
[4]	钟琪, 周雷罡, 袁铭铭, 等. HPLC法同时测定真菌竹黄胶囊中3个醌类成分的含量[J]. 药物分析杂志, 2020, 40(1): 139-144. DOI:10.16155/j.0254-1793.2020.01.18. doi: 10.16155/j.0254-1793.2020.01.18
[5]	万象义, 陈远腾. 一种新的光化学疗法药物:竹红菌甲素[J]. 科学通报, 1980, 25(24): 1148-1148.
[6]	蒋丽金. 竹红菌素的结构、性质、光化学反应及反应机制(Ⅱ):竹红菌素的反应[J]. 科学通报, 1990, 35(22): 1681-1690.
[7]	赵宁, 陈双林. 竹黄遗传多样性与竹红菌素合成和抗癌作用的研究进展[J]. 生物技术通报, 2018, 34(4): 16-23. DOI:10.13560/j.cnki.biotech.bull.1985.2018-0116. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0116
[8]	LIN X, YAN S Z, QI S S, et al. Transferrin-modified nanoparticles for photodynamic therapy enhance the antitumor efficacy of hypocrellin A[J]. Frontiers in Pharmacology, 2017, 8: 815. DOI:10.3389/fphar.2017.00815. doi: 10.3389/fphar.2017.00815 pmid: 29209206
[9]	JIA Y L. Sonodynamic action of hypocrellin B triggers cell apoptoisis of breast cancer cells involving caspase pathway[J]. Ultrasonics, 2017, 73: 154-161. DOI:10.1016/j.ultras.2016.09.013. doi: S0041-624X(16)30193-7 pmid: 27657480
[10]	祁姗姗. 竹黄的分离鉴定与竹红菌素光动力诱导凋亡的机制[D]. 南京: 南京师范大学, 2016.
[11]	林羲. 竹黄Shiraia bambusicola发酵优化及两种苝醌代谢产物的光动力抗肿瘤活性[D]. 南京: 南京师范大学, 2019.
[12]	WANG X N. Sonodynamic action of hypocrellin B on methicillin-resistant Staphylococcus aureus[J]. Ultrasonics, 2016, 65: 137-144. DOI:10.1016/j.ultras.2015.10.008. doi: 10.1016/j.ultras.2015.10.008 pmid: 26482395
[13]	YANG Y J, WANG C L, ZHUGE Y Z, et al. Photodynamic antifungal activity of hypocrellin A against Candida albicans[J]. Frontiers in Microbiology, 2019, 10: 1810. DOI:10.3389/fmicb.2019.01810. doi: 10.3389/fmicb.2019.01810
[14]	LI Y L, XU Y, LIAO Q M, et al. Synergistic effect of hypocrellin B and curcumin on photodynamic inactivation of Staphylococcus aureus[J]. Microbial Biotechnology, 2021, 14(2): 692-707. DOI:10.1111/1751-7915.13734. doi: 10.1111/1751-7915.13734
[15]	熊晓辉, 孔佳仪, 张帅, 等. 光敏剂介导光动力杀菌在食品中应用的研究进展[J]. 食品与发酵工业, 2021, 47(22): 309-318.DOI:10.13995/j.cnki.11-1802/ts.026616. doi: 10.13995/j.cnki.11-1802/ts.026616
[16]	徐倩, 任清褒, 张燕, 等. 竹红菌素的研究进展[J]. 丽水学院学报, 2021, 43(5): 43-48.
[17]	佘之蕴, 黄宝莹, 刘海卿, 等. 牛津杯法测定食品添加剂对五种益生菌的抑菌活力[J]. 食品工业, 2016, 37(1): 171-174.
[18]	中华人民共和国卫生部. 消毒技术规范(2002年版)[EB/OL].[2022-04-23]. http://law.foodmate.net/show-181974.html.
[19]	陈靖宇. 大肠杆菌的药敏试验[J]. 畜牧兽医科技信息, 2019(3): 22.
[20]	杜文, 孙春龙, 韩燕峰, 等. 竹黄菌丝体中竹红菌甲素的提取工艺[J]. 贵州大学学报(自然科学版), 2010, 27(6):70-73.DOI:10.15958/j.cnki.gdxbzrb.2010.06.015. doi: 10.15958/j.cnki.gdxbzrb.2010.06.015
[21]	邵颖, 陈安徽, 陈小洁, 等. 液体发酵竹红菌素的提取与表征[J]. 食品科学, 2016, 37(1): 139-144. DOI:10.7506/spkx1002-6630-201601025. doi: 10.7506/spkx1002-6630-201601025
[22]	李加友, 李兆兰, 焦庆才, 等. 竹黄菌发酵液萃取物的抗菌活性研究[J]. 南京中医药大学学报, 2003, 19(3):159-160. DOI:10.14148/j.issn.1672-0482.2003.03.011. doi: 10.14148/j.issn.1672-0482.2003.03.011
[23]	王婷婷, 曹秀明, 魏取福, 等. 竹红菌素纳米纤维膜的制备及其光敏抗菌性能研究[J]. 化工新型材料, 2021, 49(11): 289-293. DOI:10.19817/j.cnki.issn1006-3536.2021.11.059. doi: 10.19817/j.cnki.issn1006-3536.2021.11.059
[24]	陈毅坚, 钟文武, 杨松艳. 滇西竹黄抗菌活性研究[J]. 云南民族大学学报(自然科学版), 2010, 19(2):154-156. DOI:10.3969/j.issn.1672-8513.2010.02.024. doi: 10.3969/j.issn.1672-8513.2010.02.024
[25]	蔡宇杰, 丁彦蕊, 张大兵, 等. 竹黄菌固态发酵竹红菌素条件的研究[J]. 生物技术, 2004, 14(4):46-47.DOI:10.3969/j.issn.1004-311X.2004.04.025. doi: 10.3969/j.issn.1004-311X.2004.04.025
[26]	梁晓辉, 蔡宇杰, 廖祥儒, 等. Shiraia sp.SUPER-H168 ITS-5.8S rDNA 序列分析及产竹红菌素条件初步优化[J]. 工业微生物, 2009, 39:13-17.DOI:10.3969/j.issn.1001-6678.2009.02.003. doi: 10.3969/j.issn.1001-6678.2009.02.003

受试菌	d_CK/mm	d_竹红菌素/mm
大肠埃希氏菌8099	0	15.3±0.8
白色念珠菌 ATCC10231	0	20.0±0.6

Study on pilot-scale purification process and antibacterial activity of hypocrellin

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 26

Related Articles 15

Metrics

Comments

Recommended 0

[1]	GAO Qiuling, CHENG Wei, LI Wenlong, GE Hailong, HOU Xingqiang, SONG Ruhui, WEI Jiajie, JIA Tianshuo, CAI Xinyan. Centerline extraction algorithm of structured light streak in a complex background [J]. Shandong Science, 2024, 37(2): 65-73.
[2]	SONG Shikai, LIU Xiaoyang, GUO Yuxia, ZHAO Shuai, LI Lin. Application of green functionalized heavy calcium carbonate in rigid PVC foam sheet with high dimensional stability [J]. Shandong Science, 2024, 37(1): 59-68.
[3]	WANG Xiaokai, ZHAO Changsheng, LI Luzhen, ZHANG Bowei, LIU Xuzhen, TAN Yu. Research progress in high-salinity wastewater treatment by the freeze concentration method [J]. Shandong Science, 2023, 36(6): 121-130.
[4]	LIU Tiantian, WANG Mengfei, WU Xiaosu, JIAO Youquan, WEN Jiangli. Study on biodrying plant-fiber agricultural wastes mixed with storage sludge [J]. Shandong Science, 2023, 36(4): 134-140.
[5]	LIU Minmin, WU Yuanzheng, HU Jindong, LI Jishun, WANG Yan, YANG Hetong. Burkholderia vietnamiensis B418 labeled with red fluorescent protein and its functional stability [J]. Shandong Science, 2023, 36(1): 51-57.
[6]	GAO Jie, SONG Xiang-yun, MA Tian-yu, LIU Feng, ROMAN Kachan, WANG Xiao. Separation and purification of polyphenols from Taraxaci Herba by counter-current chromatography [J]. Shandong Science, 2022, 35(6): 1-7.
[7]	BI Yan-yan, MA Qing-wen, XU Hui, LIU Jian, LONG Rui, TANG Wei-ming, GUO Tian-tian, HAO Da-wei. Optimization of the column chromatographic purification of total flavonoids extracted from Ginkgo biloba using Box-Behnken design and response surface method [J]. Shandong Science, 2022, 35(6): 8-15.
[8]	LI Lian-wei,ZHANG Yuan-yu,YUE Zeng-you,XUE Cun-jin,FU Yu-xuan,XU Yang-feng. Extracting inland cage aquacultural areas from high-resolution remote sensing images using fully convolutional networks model [J]. Shandong Science, 2022, 35(2): 1-10.
[9]	NING Fan-sheng,LU Yan-xiang,CAO Gui-yun,MENG Zhao-qing,Yan Hui-jiao,Wang Dai-jie. Preparation of certified reference material of Ginkgolides C [J]. Shandong Science, 2022, 35(2): 18-28.
[10]	LI Qi-dong,ZHAO Jin,KONG Na,HUANG Lu-yao,PAN Shao-bin. Rapid analysis of the volatile components of Mongolian thyme herb's essential oils using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry [J]. Shandong Science, 2022, 35(1): 1-5.
[11]	FU Rui-ming,HU Jin-chun,MENG Zhao-qing,LI Qiang,CAO Gui-yun,WANG Dai-jie,YAN Hui-jiao. Preparation of certified reference material of bilobalide [J]. Shandong Science, 2022, 35(1): 6-13.
[12]	XU Yi-zhen. Analysis of volatile oil constitutes of guava leaves by solid phase microextraction [J]. Shandong Science, 2021, 34(6): 15-21.
[13]	ZHANG Tian-tian, XU Wen-wen, GUO Hong, DU Ming-yang, YU Chang-biao. Stability analysis of first-order linear convection equations with variable coefficients [J]. Shandong Science, 2021, 34(6): 112-118.
[14]	ZHANG Hua-li, ZHOU Hong-liang, SUN Jia-chang. Rapid determination of glutamic acid content in Eupolyphaga Steleophaga using an amino acid analyzer [J]. Shandong Science, 2021, 34(3): 26-32.
[15]	LIU You-bin, LI Yu-yang, LI Yan. Determination of five phenolic compounds in environmental water using solid-phase extraction coupled with high-performance liquid chromatography-tandem mass spectrometry [J]. Shandong Science, 2021, 34(3): 71-79.