HPLC-Q-TOF-MS在线筛选鉴定牡丹花中的抗氧化成分

doi:10.3976/j.issn.1002-4026.2022.05.002

Abstract

Abstract:

A high performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) combined with on-line removal of 1,1-diphenyl-2-picrylhydrazyl (DPPH) model was established for the rapid screening and identification of antioxidant active ingredients in peony flowers. Waters X-Bridge C₁₈ chromatographic column was used with acetonitrile-water (0.1% formic acid) as the mobile phase. The peony flower samples were analyzed using electrospray positive and negative ion modes. The samples separated using HPLC were mixed with free-radical solution, and the mixture was entered into HPLC-Q-TOF-MS for rapid identification. In total, 15 ingredients and 5 antioxidant compounds were identified in the negative ion mode. This method is a simple method for rapid screening and identification of antioxidant compounds in a complex system of natural products. Furthermore, it can also provide scientific basis for further studies on peony flower.

Key words: peony flower, antioxidant activity, on-line screening, HPLC-Q-TOF-MS

CLC Number:

O657.72

TIAN Xuan, LIU Yu, ZHU Wen-qing, ZHENG Zhen-Jia. Identification of antioxidant components in peony flowers through on-line screening using high performance liquid chromatography-quadrupole time of flight mass spectrometer[J].Shandong Science, 2022, 35(5): 10-15.

Figures/Tables 3

Fig.1

Fig.2

Table 1

References 30

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序号	选择离子	实验值m/z	分子式	化合物推断结果	参考文献
1	[M-H]^-	331.055 4	C₁₃H₁₆O₁₀	1-没食子酸酰葡萄糖或6-没食子酸酰葡萄糖	[23-24]
2	[M-H]^-	169.007 7	C₇H₆O₅	没食子酸	[29]
3	[M-H]^-	495.124 5	C₂₃H₂₈O₁₂	氧化芍药苷	[27]
4	[M-H]^-	609.111 8	C₂₇H₃₀O₁₆	山奈酚-3,7-二-O-葡萄糖苷或山柰酚3-O-β-D-半乳糖(2→1)-β-D-葡萄糖苷	[25-26]
5	[M-H]^-	525.128 5	C₂₄H₃₀O₁₃	牡丹皮苷-E	[30]
6	[M-H]^-	197.033 5	C₉H₁₀O₅	没食子酸乙酯	[21]
7	[M-H]^-	639.115 6	C₂₈H₃₂O₁₇	异鼠李素-3,7-二-O-葡萄糖	[25]
8	[M-H]^-	653.129 3	—	未知
9	[M-H]^-	447.064 3	C₂₁H₂₀O₁₁	木犀草素-7-O-葡萄糖苷	[25]
10	[M-H]^-	469.021 2	C₂₉H₄₂O₅	11α,12α-epoxy-3β,23-dihydroxy-30-norolean-20(29)-en-28,13β-olide	[22]
11	[M-H]^-	447.064 6	C₂₁H₂₀O₁₁	山奈酚-3-O-β-D-吡喃葡萄糖苷	[27]
12	[M-H]^-	577.118 2	C₂₇H₃₀O₁₄	芹菜素-7-O-新橘皮糖苷或芹菜素-7-O-β-D-葡萄糖-4'-O-α-L-鼠李糖苷	[27-28]
13	[M-H]^-	431.070 1	C₂₁H₂₀O₁₀	芹菜素-7-O-β-D-吡喃葡糖苷	[27]
14	[M-H]^-	545.020 0	—	未知
15	[M-H]^-	349.03 26	—	未知

Identification of antioxidant components in peony flowers through on-line screening using high performance liquid chromatography-quadrupole time of flight mass spectrometer

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