知母-黄柏配伍抗炎作用机制的网络药理学研究

doi:10.3976/j.issn.1002-4026.2021.06.006

Abstract

Abstract:

The combination of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex is a commonly used combinatorial medicine in clinical practice, which belongs to the “mutual promotion”. Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination has an anti-inflammatory effect, but the mechanism underlying this effect has not been studied well. In this study, the anti-inflammatory mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination was studied using network pharmacology. The results showed that Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination has 182 potential anti-inflammatory targets, of which 29 showed regulatory scores greater than or equal to twice the median value of the common targets of Anemarrhenae Rhizoma, Rhellodendri Chinensis Cortex, and inflammation-PTGS2, PTGS1, RXRA, ADRB2, AR, CHRM1, ADRA1B, CHRM3, F10, NOS3, etc. These targets are closely related to the mechanism of inflammation. GO(gene ontology) function showed that anti-inflammatory targets were involved in RNA polymerase Ⅱ promoter transcriptional regulation, cell proliferation, apoptosis regulation, nitric oxide biosynthesis regulation, etc. The KEGG(Kyoto encyclopedia of genes and genomes) pathway was involved in TNF, HIF-1, PI3K-Akt, FOXO, VEGF, T cell receptor, and Toll-like receptor signaling pathways and insulin resistance pathway, among others. The results suggested that the molecular mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination would be the coregulation of various signal pathways, exerting synergistic anti-inflammatory effects. The molecular mechanisms of the effect of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination studied using network pharmacology provide a scientific basis for its clinical use.

Key words: Anemarrhenae Rhizoma, Rhellodendri Chinensis Cortex, network pharmacology, inflammation, signaling pathways

CLC Number:

R285

PAN Hui-jun,ZHU Quan-gang,ZHU Cong-cong. Network pharmacology study on the anti-inflammatory mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination[J].Shandong Science, 2021, 34(6): 41-50.

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Network pharmacology study on the anti-inflammatory mechanism of Anemarrhenae Rhizoma-Rhellodendri Chinensis Cortex combination

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靶点	受知母调控得分	受黄柏调控得分	受知母-黄柏药对调控得分	靶点	受知母调控得分	受黄柏调控得分	受知母-黄柏药对调控得分
PTGS2	12	34	46	NOS2	2	10	12
PTGS1	8	26	34	PGR	6	6	12
RXRA	3	25	28	CHRNA7	3	8	11
ADRB2	6	19	25	F7	2	9	11
AR	8	15	23	HTR2A	1	10	11
CHRM1	7	15	22	ACHE	4	6	10
ADRA1B	6	14	20	DPP4	5	5	10
CHRM3	4	13	17	OPRM1	1	9	10
F10	2	15	17	NCOA1	2	7	9
NOS3	3	14	17	PIK3CG	3	6	9
PRSS1	3	12	15	PPARG	3	6	9
TOP2A	4	10	14	NR3C1	7	1	8
CHRM2	4	9	13	SLC6A3	2	6	8
ESR1	2	11	13	SLC6A4	1	7	8
F2	6	6	12

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