纳米银对小麦秸秆还田土壤中酶活性及微生物群落功能多样性的影响

doi:10.3976/j.issn.1002-4026.2021.03.012

Abstract

Abstract: An indoor cultivation system was used to study the environmental impact of nano silver on various soil characteristics, such as soil respiration, carbon-conversion-related enzyme activities, and functional diversity of microbial community. The experimental sample was set as blank control boil (untreated,CK), wheat-straw-returned soil(SW), nano silver amended soil (AgSW). The results showed that when compared with blank control soil, wheat-straw-returned soil had increased the soil respiration rate, cumulative release of CO₂was increased by 9.9%. Further, nano-silver amendment inhibited soil respiration in comparison with wheat straw treatment, and cumulative release of CO₂ was reduced by 36.8%, the activities of peroxidase, polyphenol oxidase, and cellulase were considerably reduced highest by 25.1%, 27.1%, and 14.3%, respectively. The results of microbial community level physiological characteristics revealed that nano silver significantly reduced the average well color development（AWCD） (reduced by 73.8% compared with SW) as well as microbial diversity and richness. The results show that nano silver alters the ecological process of soil organic matter decomposition by inhibiting soil microbial activity and reduces the functional diversity of microbial communities.

Key words: nano silver, wheat-straw-returned soil；soil respiration, soil enzymes；functional diversity of microbial communities

CLC Number:

CAO Xin-lei, JIANG Hao, YANG Bao-shan, JIAO Ke-qin, WANG Hui. Effect of silver nanoparticles on soil enzyme activity and functional diversity of microbial communities in wheat-straw-returned soil[J].Shandong Science, 2021, 34(3): 80-89.

References 34

1	辛琦, 章强, 程金平. 纳米银和银离子对斑马鱼胚胎早期生长发育的影响及作用机制[J]. 生态毒理学报, 2015, 10(4): 55-64. doi: 10.7524/AJE.1673-5897.20141116002
2	CHEN Z Y, YANG P, YUAN Z G, et al. Aerobic condition enhances bacteriostatic effects of silver nanoparticles in aquatic environment: An antimicrobial study on Pseudomonas aeruginosa[J]. Scientific Reports, 2017, 7: 7398. doi: 10.1038/s41598-017-07989-w
3	YI F, CHEN G Q, ZENG G M, et al.Influence of cysteine and bovine serum albumin on silver nanoparticle stability, dissolution, and toxicity to Phanerochaete chrysosporium[J]. RSC Advances, 2016, 6(108): 106177-106185. doi: 10.1039/c6ra23675h
4	周东美. 纳米Ag粒子在我国主要类型土壤中的迁移转化过程与环境效应[J]. 环境化学, 2015, 34(4):605-613. doi: 10.7524/j.issn.0254-6108.2015.04.20150107
5	ZHANG H L, HUANG M, ZHANG W H, et al.Silver nanoparticles alter soil microbial community compositions and metabolite profiles in unplanted and cucumber-planted soils[J]. Environmental Science & Technology, 2020, 54(6): 3334-3342. doi: 10.1021/acs.est.9b07562
6	衣俊, 黄俊, 程金平. 纳米银在水环境中的环境行为和毒性效应研究进展[J]. 生态毒理学报, 2015, 10(1): 101-109. doi: 10.7524/AJE.1673-5897.20140314003
7	袭著革, 林治卿. 纳米尺度物质对生态环境的影响及其生物安全性的研究进展与展望[J]. 生态毒理学报, 2006, 1(3): 203-208. doi: 10.3969/j.issn.1673-5897.2006.03.002
8	SHIN Y J , KWAK J I , AN Y J . Evidence for the inhibitory effects of silver nanoparticles on the activities of soil exoenzymes[J]. Chemosphere, 2012, 88(4):524-529. doi: 10.1016/j.chemosphere.2012.03.010
9	RAHMATPOUR S, SHIRVANI M, MOSADDEGHI M R, et al. Dose-response effects of silver nanoparticles and silver nitrate on microbial and enzyme activities in calcareous soils[J]. Geoderma, 2017, 285: 313-322. doi: 10.1016/j.geoderma.2016.10.006
10	王秋双, 戚兴超, 申天琳, 等. 纳米银与石墨烯对土壤微生物及土壤酶的影响[J]. 环境科学学报, 2017, 37(8): 3149-3157. doi: 10.13671/j.hjkxxb.2017.0013
11	MEIER M J, DODGE A E, SAMARAJEEWA A D, et al. Soil exposed to silver nanoparticles reveals significant changes in community structure and altered microbial transcriptional profiles[J]. Environmental Pollution, 2020, 258: 113816. doi: 10.1016/j.envpol.2019.113816
12	SAMARAJEEWA A D, VELICOGNA J R, PRINCZ J I, et al. Effect of silvernano-particles on soil microbial growth, activity and community diversity in a sandy loam soil[J]. Environmental Pollution, 2017, 220: 504-513. doi: 10.1016/j.envpol.2016.09.094
13	舒昆慧, 张丽, 伍玲丽, 等. 纳米银对四种不同性质土壤微生物量及酶活性的影响[J]. 农业环境科学学报, 2018, 37(5): 907-914. doi: 10.11654/jaes.2017-1325
14	SILLEN W M A, THIJS S, ABBAMONDI G R, et al. Effects of silver nanoparticles on soil microorganisms and maize biomass are linked in the rhizosphere[J]. Soil Biology and Biochemistry, 2015, 91: 14-22. doi: 10.1016/j.soilbio.2015.08.019
15	GRUN A L, STRASKRABA S, SCHULZ S,et al. Long-term effects of environmentally relevant concentrations of silver nanoparticles on microbial biomass, enzyme activity, and functional genes involved in the nitrogen cycle of loamy soil[J]. Journal of Environmental Sciences, 2018,69:12-22.
16	JOS＇KO I, OLESZCZUK P,DOBRZYN＇SKA J, et al. Long-term effect of ZnO and CuO nanoparticles on soil microbial community in different types of soil[J]. Geoderma, 2019, 352: 204-212. doi: 10.1016/j.geoderma.2019.06.010
17	DE OCA-VSQUEZ G M, SOLANO-CAMPOS F, VEGA-BAUDRIT J R, et al. Organic amendments exacerbate the effects of silver nanoparticles on microbial biomass and community composition of a semiarid soil[J]. Science of The Total Environment, 2020, 744: 140919. doi: 10.1016/j.scitotenv.2020.140919
18	郭瑞华, 靳红梅, 常志州, 等. 秸秆还田模式对土壤有机碳及腐植酸含量的影响[J]. 农业环境科学学报, 2017, 36(4): 727-733. DOI: 10.11654/jaes.2016-1364.
19	WANG H, HU G Q, XU W H, et al.Effects of nitrogen addition on soil organic carbon mineralization after maize stalk addition[J]. European Journal of Soil Biology, 2018, 89: 33-38. doi: 10.1016/j.ejsobi.2018.10.002
20	ALOTAIBI H S, USMAN A R, ABDULJABBAR A S, et al.Carbon mineralization and biochemical effects of short-term wheat straw in crude oil contaminated sandy soil[J]. Applied Geochemistry, 2018, 88: 276-287. doi: 10.1016/j.apgeochem.2017.02.017
21	RASHID M I, SHAHZAD T, SHAHID M, et al. Zinc oxide nanoparticles affect carbon and nitrogen mineralization of Phoenix dactylifera leaf litter in a sandy soil[J]. Journal of Hazardous Materials, 2017, 324: 298-305. doi: 10.1016/j.jhazmat.2016.10.063
22	ZHAO S C, ZHANG S Q. Linkages between straw decomposition rate and the change in microbial fractions and extracellular enzyme activities in soils under different long-term fertilization treatments[J]. PLoS One, 2018, 13(9): e0202660. doi: 10.1371/journal.pone.0202660
23	严昶升. 土壤肥力研究方法[M]. 北京：农业出版社, 1988.
24	关松荫. 土壤酶及其研究法[M]. 北京：农业出版社, 1986.
25	杜毅飞, 方凯凯, 王志康,等. 生草果园土壤微生物群落的碳源利用特征[J]. 环境科学, 2015, 36(11):4260-4267. doi: 10.13227/j.hjkx.2015.11.042
26	SAMARAJEEWA A D , VELICOGNA J R , SCHWERTFEGER D M , et al. Effect of silver nanoparticle contaminated biosolids on the soil microbial community[J]. Nanoimpact, 2019：14. doi: 10.1016/j.impact.2019.100157
27	RAHMATPOUR S, SHIRVANI M, MOSADDEGHI M R, et al. Dose-response effects of silver nanoparticles and silver nitrate on microbial and enzyme activities in calcareous soils[J]. Geoderma, 2017, 285: 313-322. doi: 10.1016/j.geoderma.2016.10.006
28	JIANG J P, WU L H, LI N, et al. Effects of multiple heavy metal contamination and repeated phytoextraction by Sedum plumbizincicola on soil microbial properties[J]. European Journal of Soil Biology, 2010, 46(1): 18-26. doi: 10.1016/j.ejsobi.2009.10.001
29	TCHOUNWOU P B , YEDJOU C G , PATLOLLA A K , et al. Heavy metal toxicity and the environment[J]. Experientia Supplementum, 2012, 101: 133-164. doi: 10.1007/978-3-7643-8340-4_6
30	GUPTA P, DIWAN B. Bacterial exopolysaccharide mediated heavy metal removal: a review on biosynthesis, mechanism and remediation strategies[J]. Biotechnology Reports, 2017, 13: 58-71. doi: 10.1016/j.btre.2016.12.006
31	彭程. 纳米银对湿地土壤酶活性影响的研究[D]. 南京: 东南大学, 2017.
32	XU C, PENG C, SUN L J, et al.Distinctive effects of TiO2 and CuO nanoparticles on soil microbes and their community structures in flooded paddy soil[J]. Soil Biology and Biochemistry, 2015, 86: 24-33. doi: 10.1016/j.soilbio.2015.03.011
33	MA H B, WALLIS L K, DIAMOND S, et al. Impact of solar UV radiation on toxicity of ZnO nanoparticles through photocatalytic reactive oxygen species (ROS) generation and photo-induced dissolution[J]. Environmental Pollution, 2014, 193: 165-172. doi: 10.1016/j.envpol.2014.06.027
34	RODRIGUEZ-YANEZ Y, MUNOZ B, ALBORES A. Mechanisms of toxicity by carbon nanotubes[J]. Toxicology Mechanisms and Methods, 2013, 23(3): 178-195. doi: 10.3109/15376516.2012.754534

[1]	WANG Yi-lian, HUANG Ding-li, WEI Yan-li, LI Hong-mei, YANG Hong-tong, LI Ji-shun. Soil conditioner and Trichoderma LTR-2 combined application for remediation of continuous cropping obstacle soil in Brassica chinensis L. [J]. Shandong Science, 2022, 35(6): 74-79.
[2]	YANG Qian,YANG Bao-shan,QIN Guang-hua,WANG Hui,SHI Jia-xing,LIU Xue-ting. Salt tolerance evaluation and selection of salt tolerance indexes for new varieties of willow seedlings [J]. Shandong Science, 2022, 35(3): 107-114.
[3]	ZHANG Teng-fei, HUANG Yu-jie, JI Lei, WANG Jia-ning. Proceedings of bioremediation technology for petroleum contaminated soil [J]. Shandong Science, 2020, 33(5): 106-112.
[4]	JI Lei, CHEN Guan-hong, FU Xiao-wen, HUAGN Yu-jie, WANG Jia-ning, ZHANG Qiang. Pilot study on bioenzyme degradation to pentachlorodiphenyl [J]. SHANDONG SCIENCE, 2016, 29(6): 94-97.
[5]	HUANG Yujie, QIU Weizhong, GAO Yongchao, KONG Xue, ZHANG Wen, WANG Jianing. Isolation, identification and optimization of growth conditions of a strain of phenanthrene degrading bacteria [J]. SHANDONG SCIENCE, 2015, 28(2): 75-80.
[6]	ZHANG Qiang,ZHENG Liwen, KONG Xue, CHEN Guanhong,ZHANG Jingyu,WANG Jianing. Enhanced bioremediation of additive on petroleum contaminated soil [J]. SHANDONG SCIENCE, 2015, 28(1): 78-81.

Effect of silver nanoparticles on soil enzyme activity and functional diversity of microbial communities in wheat-straw-returned soil

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References 34

Related Articles 6

Metrics

Comments

Recommended 0