高效凝胶过滤色谱法测定菊糖聚合度

doi:10.3976/j.issn.1002-4026.2020.04.001

Abstract

Abstract: Inulin is a type of natural macromolecular polysaccharide and its physiological activity and processing characteristics are affected by the degree of polymerization.In this study,OHpak SB-802.5 HQ was used as the chromatographic column.The injection volume was 20 μL,the column temperature was 30 ℃,the mobile phase was 0.3 moL/L NaNO₃ solution,and the flow rate was 0.3 mL/min.The cubic equation curve was fitted using GPC software.The molecular weights of nine samples were measured using high-performance gel chromatography and the degree of polymerization was calculated. Results showed that the inulin polymerization degree of inulin of nine samples ranged between 10 and 29 and the polymerization degree of different batches and varieties of the same raw material were different. The proposed method provides a reference for analyzing the structural properties and controlling the product quality of inulin.

Key words: inulin, degree of polymerization, high performance gel filtration chromatography, polysaccharide content, relative molecular weight

CLC Number:

O657.7

LI Ling-yu, LI Yu-chen, ZUO Zhao-he, TIAN bao-lan, ZHENG Zhen-jia. Development of high performance gel chromatography for the determination of inulin polymerization[J].Shandong Science, 2020, 33(4): 1-6.

References 23

1	PANCHEV I, DELCHEV N, KOVACHEVA D, et al. Physicochemical characteristics of inulins obtained from Jerusalem artichoke (Helianthus tuberosus L.)[J]. European Food Research and Technology, 2011, 233(5): 889-896. DOI:10.1007/s00217-011-1584-8.
2	APOLINRIO A C, DE LIMA DAMASCENO B P G, DE MACDO BELTRO N E, et al. Inulin-type fructans: a review on different aspects of biochemical and pharmaceutical technology[J]. Carbohydrate Polymers, 2014, 101: 368-378. DOI:10.1016/j.carbpol.2013.09.081.
3	胡秀沂, 邱树毅, 王慧, 等. 新鲜菊芋的预处理及微波辅助提取菊粉的研究[J]. 食品工业科技, 2007, 28(4): 150-152, 155. DOI:10.3969/j.issn.1002-0306.2007.04.041.
4	DAN A, GHOSH S, MOULIK S P.Physicochemistry of the interaction between inulin and alkyltrimethylammonium bromides in aqueous medium and the formed coacervates[J]. The Journal of Physical Chemistry B, 2009, 113(25): 8505-8513. DOI:10.1021/jp902641d.
5	RODRIGUEZ FURLN L T, PADILLA A P, CAMPDERRS M E. Development of reduced fat minced meats using inulin and bovine plasma proteins as fat replacers[J]. Meat Science, 2014, 96(2): 762-768. DOI:10.1016/j.meatsci.2013.09.015.
6	胡德亮, 陈有容, 齐凤兰, 等. 菊芋作为双歧杆菌促生长因子的研究[J]. 食品科学, 2001, 22(4): 49-52. DOI:10.3321/j.issn:1002-6630.2001.04.012.
7	VERMA A, SHUKLA G. Administration of prebiotic inulin suppresses 1,2-dimethylhydrazine dihydrochloride induced procarcinogenic biomarkers fecal enzymes and preneoplastic lesions in early colon carcinogenesis in Sprague Dawley rats[J]. Journal of Functional Foods, 2013, 5(2): 991-996. DOI:10.1016/j.jff.2013.02.006.
8	陈书攀, 何国庆, 谢卫忠, 等. 菊粉对面团流变性及面条质构的影响[J]. 中国食品学报, 2014, 14(7): 170-175. DOI:10.16429/j.1009-7848.2014.07.028.
9	胡娟. 菊芋菊糖的纯化及在植脂掼奶油中的应用[D]. 无锡: 江南大学, 2007.
10	PADALINO L, COSTA C,CONTE A, et al. The quality of functional whole-meal durum wheat spaghetti as affected by inulin polymerization degree[J]. Carbohydrate Polymers, 2017, 173: 84-90. DOI:10.1016/j.carbpol.2017.05.081.
11	LUO D L, LI Y, XU B C, et al. Effects of inulin with different degree of polymerization on gelatinization andretrogradation of wheat starch[J]. Food Chemistry, 2017, 229: 35-43. DOI:10.1016/j.foodchem.2017.02.058.
12	MILANI E, KOOCHEKI A, GOLIMOVAHHED Q A. Extraction of inulin from Burdock root (Arctium lappa) using high intensity ultrasound[J]. International Journal of Food Science & Technology, 2011, 46(8): 1699-1704. DOI:10.1111/j.1365-2621.2011.02673.x.
13	张惟杰. 糖复合物生化研究技术[M]. 杭州: 浙江大学出版社, 1999.
14	成跃祖. 凝胶渗透色谱法的进展及其应用[M]. 北京: 中国石化出版社, 1993.
15	冯鑫, 夏宇, 陈贵堂, 等. 生姜皮多糖的分离纯化及其结构组成分析[J]. 食品科学, 2017, 38(6): 185-190. DOI:10.7506/spkx1002-6630-201706029.
16	LIAO W Z, LU Y J, FU J N, et al. Preparation andcharacterization of Dictyophora indusiata Polysaccharide-zinc complex and its augmented antiproliferative activity on human cancer cells[J]. Journal of Agricultural and Food Chemistry, 2015, 63(29): 6525-6534. DOI:10.1021/acs.jafc.5b00614.
17	张忠华. 菊芋多糖的提取、分离、纯化及分析研究[D]. 长春: 吉林大学, 2015.
18	昌盛, 王丽娜, 李妍, 等. 国内六省份菊芋多糖含量比较[J]. 食品工业, 2012, 33(9): 127-129
19	SAHAA K, BREWER C F. Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method[J]. Carbohydrate Research, 1994, 254: 157-167. DOI:10.1016/0008-6215(94)84249-3
20	李琬聪, 李青, 董方, 等. 菊糖分子量的高效凝胶过滤色谱检测方法及其在贮藏过程中的变化研究[J]. 中国科学:生命科学, 2016, 46(9): 1107-1112.
21	CHEN J C, ZHANG X, HUO D, et al. Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus[J]. Carbohydrate Polymers, 2019, 215: 307-315. DOI:10.1016/j.carbpol.2019.03.099.
22	李丹丹. 牛蒡菊糖的制备、对双歧杆菌的增殖及应用研究[D]. 无锡: 江南大学, 2008.
23	LI D D, KIM J M, JIN Z Y, et al. Prebiotic effectiveness of inulin extracted from edible burdock[J]. Anaerobe, 2008, 14(1): 29-34. DOI:10.1016/j.anaerobe.2007.10.002.

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Development of high performance gel chromatography for the determination of inulin polymerization

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