SHANDONG SCIENCE ›› 2018, Vol. 31 ›› Issue (5): 43-47.doi: 10.3976/j.issn.1002-4026.2018.05.008
• Biosensors • Previous Articles Next Articles
ZHAO Kai, GE Jing-hua, WANG Hai
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Abstract:
Different specific growth rates lead to different metabolic fluxes in Pichia pastoris, further resulting in different levels of enzyme production. In order to obtain optimum specific growth rate for the production of phytase by Pichia pastoris, the optimal fermentation conditions of recombinant Pichia pastoris producing phytase in 50 L fermentation tank were studied in this paper. The results showed that specific growth rate was adjusted to 0.02 h-1 by controlling the flow of methanol, by the end of fermentation, the phytase activity got to be 17 445 U/mL, with 69.7 % higher than the control. It suggests that the specific growth rate is the most favorable for the production of phytase by fermentation.
Key words: specific growth rate, Pichia pastoris, fermentation optimization, phytase
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ZHAO Kai, GE Jing-hua, WANG Hai. Effects of different specific growth rates on phytase production by Pichia pastoris fermentation[J].SHANDONG SCIENCE, 2018, 31(5): 43-47.
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