Abstract:

To establish the quality control method of Xiaochuan Emplastrum,Sinapis Semen, Corydalis Rhizoma, Asari Radix et Rhizoma and Piperis Longi Fructus in the Xiaochuan Emplastrum were qualitatively analyzed by TLC. The content of sinapine thiocyanate in sinapis semen was determined by HPLC. The chromatographic column was Phenomenex Geminin C18 column（150 mm×4.6 mm, 5 μm）. The mobile phase was acetonitrile0.08 mol/L potassium phosphate monobasic (13∶87,V/V) at the flow rate of 1.0 mL/min, the detection wavelength of 326 nm, the column temperature of 25 ℃ and the sample volume of 20 μL. The results revealed that the spots in the TLC identification of Sinapis Semen, Corydalis Rhizoma, Asari Radix et Rhizoma and Piperis Longi Fructus were clear, well separated and without interference by the negative control. The calibration curve of sinapine thiocyanate showed good linearity in the range of 0.84～8.4 μg (r=0.999 7). The average recovery was 99.58 % (n=6,reltive standard deviatior is 0.37 %). This method is rapid, accurate, reliable, and specific, thus it can be used for the effective quality control of the Xiaochuan Emplastrum.

Key words: Xiaochuan Emplastrum, TLC, sinapine thiocyanate, quality standard, HPLC