荧光法测定叶绿素a的影响因素及其数据校正

doi:10.3976/j.issn.1002-4026.2017.03.002

Abstract

Abstract:

The impacts of turbidity and temperature on the determination of chlorophyll a in the sea water with field fluorescence method were studied in this paper. The chlorophyll a concentration of Chlorella vulgaris at different temperature and turbidity gradients was determined by visible spectrophotometer and the field fluorescence respectively, to explore the influence rule and the method for correction. Results reveal that temperature has no significant effects on the concentration of chlorophyll a in the range of 7 ~ 30 ℃, while turbidity has significant influence on the determination of chlorophyll a in the range of 0 ~ 1 000 NTU, which should be modified by the following method: The true value equals to measured value minus 0.017 4 times’ turbidity value (NTU).

Key words: turbidity, chlorophyll a, temperature, fluorescence

CLC Number:

P714

ZHANG Li,GUO Cui-lian, ZHANG Shu-wei, WU Ning, WANG Xiao-hong, ZHANG Tian-peng, WANG Zhao-yu. The influence factors of fluorescence determination of chlorophyll a and the data correction for them[J].SHANDONG SCIENCE, 2017, 30(3): 8-11.

References

［1］周虹丽, 朱建华, 杨安安. 两种方法测量水体叶绿素a浓度的比较研究［J］. 海洋技术, 2013, 32(4): 4448.
［2］国家环保总局水和废水监测分析方法编委会. 水和废水监测分析方法［M］. 4 版.北京: 中国环境科学出版社, 2002: 670670.
［3］赵洋甬, 沈碧君, 胡建林, 等. 浊度对荧光法测定叶绿素a的影响［C］ //中国环境科学学会学术年会论文集. 北京：［s.n］，2013.
［4］WRIGHT S W, JEFFREY S W, MANTOURA R F C, et.al. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton ［J］. Marine Ecology Progress Series, 1991, 77: 183196.

［5］LEFEVRE F, CHALIFOUR A, YU L, et al. Algal fluorescence sensor integrated into a microfluidic chip for water pollutant detection［J］. Lab on a Chip, 2012, 12(4): 787793.
［6］ LEEUW T, BOSS E S, WRIGHT D L. In situ measurements of phytoplankton fluorescence using low cost electronics ［J］. Sensors, 2013, 13(6): 78727883.
［7］ WANG J S, SUN J Y, SONG Y X,et.al. A labelfree microfluidic biosensor for activity detection of single microalgae cells based on chlorophyll fluorescence ［J］. Sensors, 2013, 13(12): 1607516089.
［8］董大圣, 程群, 陈世哲, 等. 原位叶绿素a和浊度传感器设计［J］. 仪表技术与传感器, 2012, 10: 1516.
［9］尚云, 黄启俊, 常胜, 等. 叶绿素荧光检测电路设计［J］. 测控技术, 2015, 34(3): 912.
［10］刘建华. 荧光法测定水体中叶绿素含量影响因素的研究［D］. 河北: 河北科技大学, 2014.
［11］张武，赵洋甬，陈元.叶绿素自然降解速率［J］.福建分析测试，2009,18（4）：8183.
［12］中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB 17378.7—2007海洋监测规范,第7部分,近海污染生态调查和生物监测［S］. 北京：中国标准出版社，2008.
［13］吕鹏翼, 崔建升, 段莉丽. 浊度对荧光光谱法测定水体中叶绿素a的影响［J］. 理化检验(化学分册), 2015, 51(6): 741744.

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The influence factors of fluorescence determination of chlorophyll a and the data correction for them

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