Abstract:

The specific chromatogram of flavonoids in Radix Scutellariae was established by HPLC in this paper. The column filling, eluent and the elution condition of solid phase extraction were optimized using the recovery rates and specific spectral similarity of six main characteristic peaks in HPLC specific chromatogram as indexes. Experimental results confirmed that C18 solid phase extraction (SPE) possessed the highest recovery rates of six components. The SPE column was rinsed with methanol0.3% formic acid（20∶80，V/V）, and then was eluted finally with methanol (containing 0.3% formic acid). After being purified, the recovery rates of six components were all over 89%, and the similarity of characteristic chromatogram was higher than 0.98. Based on HPLC characteristic chromatogram, the optimized purification process of flavonoids in Radix Scutellariae with solid phase extraction can ensure that the main effective components remain consistent before and after purification of Radix Scutellariae.

Key words: Radix Scutellariae, solid phase extraction, flavonoids, HPLC specific chromatogram