高速逆流色谱分离纯化番泻叶中的苷类化合物

doi:10.3976/j.issn.1002-4026.2017.01.003

SHANDONG SCIENCE ›› 2017, Vol. 30 ›› Issue (1): 15-19.doi: 10.3976/j.issn.1002-4026.2017.01.003

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Separation and purification of glycosidic compounds from Folium Sennae by high-speed counter-current chromatography

Duan Wen-juan¹, WU Qiu-xia^{1, 2}, Li Yue¹, Yang Guo-hong¹, SHI Xin-gang¹, Sun Wei-hong², WANG Xiao^1*

1.Shandong Provincial Key Laboratory for TCM Quality Control Technology, Shandong Analysis and test Center, Jinan 250014, China; 2. School of Food and Biology Engineering, Jiangsu University, Zhenjiang 212013, China

Received:2016-11-02 Online:2017-02-20 Published:2017-02-20

Abstract

Abstract:

Four glycosidic compounds were rapidly separated from the nbutyl alcohol of Folium Sennae by high-speed counter-current chromatography with the solvent system of nbutanolwater (9:10, V/V). The flow rate is 2.0 mL/min, the detection wavelength is 280 nm, and the rotate speed rating is 860 r/min. Separated from the nbutyl alcohol extracts (200 mg) of Folium Sennae, their structures were identified as apigenin-6, 8-di-C-glycoside (10 mg), kaempferol-3-O-β-D gentiobioside (9 mg), isorhamnetin-3-O-β-D gentiobioside (15 mg), and sennoside B (5 mg) with the purities were respectively 97.6%, 98.9%, 98.3%, and 99.3%. The results show that this method is simple and efficient, which is suitable for the separation and purification of glycosidic compounds from Folium Sennae.

Key words: highspeed countercurrent chromatography, separation and purification, Folium Sennae, glycosidic compounds

CLC Number:

R284.2

Duan Wen-juan, WU Qiu-xia, Li Yue, Yang Guo-hong, SHI Xin-gang, Sun Wei-hong, WANG Xiao. Separation and purification of glycosidic compounds from Folium Sennae by high-speed counter-current chromatography[J].SHANDONG SCIENCE, 2017, 30(1): 15-19.

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Separation and purification of glycosidic compounds from Folium Sennae by high-speed counter-current chromatography

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