Abstract: We address dosedependent developmental toxicity of isoniazid (INH) to zebrafish. We respectively process 2hpf zebrafish embryo with different concentrations of INH, and observe the variations of the mortality of zebrafish larva and embryo, hatching rate, malformation rate, body length and morphology. Acute toxicity experiment shows that INH has significant developmental toxicity to zebrafish, and can cause developmental delay and malfunction of zebrafish. Compared with the control group, lower doses (<6 mmol/L) of INH have no developmental toxicity to zebrafish, while higher doses (≥32 mmol/L) of INH significantly inhibit hatching rate of embryos, and have obvious embryonic death and teratogenic effects. Mortality and malformation rates of zebrafish both increase with the increase of drug delivery dosage and time extension. For this experiment, the largest concentration of INH nontoxic effect is 2 mmol/L.

Key words: isoniazid, zebrafish, developmental toxicity