Abstract:

       We construct a subcritical water extraction method for baicalin, wogonoside，baicalein and wogonin from Scutellaria baicalenses. We also investigate the impacts of temperature, extraction time and water loadings on extraction yield and compare it with methanol extraction method. The extraction yield of baicalin and wogonoside is higher at extraction temperature of 160 ℃, extraction time of 60 min. The extraction yield of baicalein and wogonin is higher at extraction temperature of 110 ℃, extraction time of 10 min.Extraction time and extraction solvent consumption remarkbly decrease for the subcritical water extraction method, so the pollution of organic solvent is avoided.

Key words: subcritical water, Scutellaria baicalenses, baicalin, baicalein, wogonin, wogonoside