高速逆流色谱分离纯化波棱瓜子中化学成分

doi:10.3976/j.issn.1002-4026.2014.01.007

J4 ›› 2014, Vol. 27 ›› Issue (1): 40-44.doi: 10.3976/j.issn.1002-4026.2014.01.007

• Traditional Chinese medicine and natural active products • Previous Articles Next Articles

Separation and purification of chemical constituents in Herpetospermum pedunculosum seeds by highspeed countercurrent chromatography

HANG Wei^1,2, SONG Teng-Fei², DUAN Wen-Juan², WANG Xiao², GENG Yan-Ling², YANG Bing-Tian³, ZHANG Qin-De⁴

1.College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2.Shandong Analysis and Test Center, Jinan 250014, China; 3.Three peak biological engineering technology Co. Ltd., Jinan 250014, China; 4.Shandong College of Traditional Chinese Medicine, Yantai 264000, China

Received:2014-04-30 Published:2014-02-20 Online:2014-02-20

Abstract

Abstract:

We originally employed high speed countercurrent chromatography (HSCCC) to separate and purify chemical constituents in ethyl acetate dissolved Herpetospermum pedunculosum seeds. We separated two highpurity chemical compounds with petroleum etherethyl acetatemethanolwater (volume ratio of 2:6:3:5) as solvent, upper organic stationary phase and lower aqueous mobile phase, flow rate of 2 mL/min and rotation speed of 850 r/min. Their HPLC detected purities were more than 95%. The method is simple, rapid, solventsaving and can rapidly and effectively separate and purify the chemical constituents in Herpetospermum pedunculosum seeds.

Key words: highspeed countercurrent chromatography, Herpetospermum pedunculosum seed, separation and purification

CLC Number:

R284.2

HANG Wei, SONG Teng-Fei, DUAN Wen-Juan, WANG Xiao, GENG Yan-Ling, YANG Bing-Tian, ZHANG Qin-De. Separation and purification of chemical constituents in Herpetospermum pedunculosum seeds by highspeed countercurrent chromatography[J].J4, 2014, 27(1): 40-44.

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Separation and purification of chemical constituents in Herpetospermum pedunculosum seeds by highspeed countercurrent chromatography

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