Abstract:

Rats were intragastrically administered with isochlorogenic acid A to identify the metabolites in their feces, urine, and plasma. Rat feces, urine, and plasma samples were collected at different time points after intragastric administration of isochlorogenic acid A at a dose of 20 mg/kg. The treated samples were analyzed via UHPLC-Q-Exactive Orbitrap MS. According to the relative retention time, mass/charge ratio, and characteristic fragment ions, 10 metabolites including the parent compound were identified. Among them, four metabolites from feces and six from urine samples were obtained. the main metabolic pathways included hydrogenation, methylation, glucuronidation, and sulfatation. The study identified the metabolites of isochlorogenic acid A in rats, clarified the pharmacodynamic material basis of isochlorogenic acid A, and provided the material basis for further research and development.

Key words: isochlorogenic acid A, Orbitrap MS technique, metabolites, metabolic pathways, intragastric administration