丹参植株邻苯二甲酸与立枯丝核菌菌丝生长互作研究

doi:10.3976/j.issn.1002-4026.2020.05.004

Abstract

Abstract: To investigate the continuous cropping barrier of Salvia miltiorrhiza Bge.caused by the interaction between phthalic acid and Rhizoctonia solani mycelium growth, the source of phthalic acid was determined by gas chromatography. The effect of phthalic acid on mycelia growth and its critical concentration were studied by laboratory mycelia growth inhibition experiment. The content of phthalic acid in the culture system before and after the growth of hyphae was determined using HPLC. The results showed six types of phthalate esters in the above ground part of S. miltiorrhiza, with a total relative content of 8.264%. The under ground part contains five types of phthalate esters, with a total relative content of 1.069%. Phthalic acid concentration promoted in low concentration and inhibited high concentration in the growth of R. solani. When the concentration was 0.1 mg/mL, the number of bacterial colonies of R. solani was the highest and the colony diameter was the largest. Under the same culture conditions, the addition of phthalic acid promoted the growth and reproduction of R. solani, but its content did not decrease, proving that phthalic acid did not provide C source for the growth of R. solani but acted as a catalyst. This study aimed to provide a new method and theoretical basis for overcoming the continuous cropping barrier of S. miltiorrhiza.

Key words: Salvia miltiorrhiza Bge., phthalic acid, origin identification, Rhizoctonia solani, growth of interactions

CLC Number:

R931.2

FENG Jing, ZHOU Bing-qian, LIU Qian, WANG Xiao, LU Heng, GUO Lan-ping, LIU Wei, SHI Guo-yu. Study on the interaction between phthalic acid and Rhizoctonia solani mycelium growth of Salvia miltiorrhiza Bge.[J].Shandong Science, 2020, 33(5): 34-42.

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Study on the interaction between phthalic acid and Rhizoctonia solani mycelium growth of Salvia miltiorrhiza Bge.

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