绿色木霉LTR-2的ATMT插入突变及5,6-二氢-6-戊基-2H-吡喃-2-酮高产菌株的筛选

J4 ›› 2010, Vol. 23 ›› Issue (6): 39-43.

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nsertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of HighYield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one

HU Jin-Dong¹, ZHOU Hong-Zi, ZHANG Yan-Jie², YANG He-Tong^1,2,3*, CHEN Kai¹, LI Ji-Shun¹

1.Biotechnology Center of Shandong Academy of Sciences, Jinan 250014, China;
2.School of Life Sciences, Shandong University of Techology, Zibo 255049, China;
3.Biology Institute of Shandong Academy of Sciences, Jinan 250014, China

Received:2010-06-22 Online:2010-12-20 Published:2010-12-20

Abstract

Abstract:

We performed insertional mutation for Trichoderma viride LTR-2 by Agrobacteria tuefaxiensmediated transformation and obtained 400 mutants. We selected six highyield mutants from these four hundred mutants by powder bacteriostatic circle method. These mutants were identified and analyzed by gas chromatography and mass spectrometric detection. The content of 5,6-dihydro-6-pentyl-2H-pyran-2-one is 2.62 mg/g, which is produced by one mutant T-54 in a PDA plate and is nine times as that by LTR-2.

Key words: insertional mutation, 5,6-dihydro-6-pentyl-2H-pyran-2-one, GC/MS

CLC Number:

S432.2

HU Jin-Dong, ZHOU Hong-Zi, ZHANG Yan-Jie, YANG He-Tong, CHEN Kai, LI Ji-Shun. nsertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of HighYield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one[J].J4, 2010, 23(6): 39-43.

nsertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of HighYield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one

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