J4 ›› 2010, Vol. 23 ›› Issue (6): 39-43.

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nsertional Mutation of Trichoderma viride LTR-2 by ATMT and Screening of HighYield Strains of 5,6-dihydro-6-pentyl-2H-pyran-2-one

 HU Jin-Dong1, ZHOU Hong-Zi, ZHANG Yan-Jie2, YANG He-Tong1,2,3*, CHEN Kai1, LI Ji-Shun1   

  1. 1.Biotechnology Center of Shandong Academy of Sciences, Jinan 250014, China;
    2.School of Life Sciences, Shandong University of Techology, Zibo 255049, China;
    3.Biology Institute of Shandong Academy of Sciences, Jinan 250014, China
  • Received:2010-06-22 Published:2010-12-20 Online:2010-12-20

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Abstract:

        We performed insertional mutation for Trichoderma viride LTR-2 by Agrobacteria tuefaxiensmediated transformation and obtained 400 mutants. We selected six highyield mutants from these four hundred mutants by powder bacteriostatic circle method. These mutants were identified and analyzed by gas chromatography and mass spectrometric detection. The content of 5,6-dihydro-6-pentyl-2H-pyran-2-one is 2.62 mg/g, which is produced by one mutant T-54 in a PDA plate and is nine times as that by LTR-2.

Key words: insertional mutation, 5,6-dihydro-6-pentyl-2H-pyran-2-one, GC/MS

CLC Number: 

  • S432.2

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