马钱子苷对神经干细胞的增殖、存活和分化的调节作用

doi:10.3976/j.issn.1002-4026.2021.05.002

Abstract

Abstract: The present study aimed to investigate the regulatory effects of different concentrations of Loganin on the proliferation, survival, and differentiation of neural stem cells (NSCs) and its related molecular mechanisms. NSCs were isolated and cultured from the brain of adult mouse. The effects of Loganin on the proliferation, survival, and differentiation of NSCs were determined using different concentrations of Loganin.The results showed that the number and diameter of adult mouse NSCs significantly increased compared with those of control mouse after culturing in medium and high concentrations of Loganin for 5 days and 7 days (P<0.05). Medium and high concentrations of Loganin could promote the mitosis of NSCs. Low-dose Loganin treatment significantly promoted the differentiation of NSCs (P<0.01) and increased the number and proportion of neurons and astrocytes (P<0.01). However, medium and high concentrations of Loganin inhibited the differentiation of NSCs (P<0.05). Furthermore, high concentrations of Loganin reduced the number of neurons (P<0.05). These results suggest that a high concentration of Loganin could promote the survival of NSCs and enhance their proliferative ability by promoting the mitosis of NSCs. On the other hand, a low concentration of Loganin could promote the differentiation of NSCs and is beneficial for neurogenesis and oligodendrocyte regeneration. These results lay the theoretical and experimental foundation for research on NSCs for the treatment of central nervous system diseases.

Key words: Loganin, neural stem cells, proliferation, survival, differentiation, neurogenesis

CLC Number:

R284.2

ZHANG Jin-qiang, YI Sai-ni, SHEN Lin-min, LI Liang-yuan, XIAO Cheng-hong, TANG Xin, LIU Qin, SU Da-peng, XU Chun-yun, ZHOU Tao. Regulatory effects of Loganin on the proliferation, survival, and differentiation of nueral stem cells[J].Shandong Science, 2021, 34(5): 8-15.

References 12

1	张敏娜, 张一兵, 王光辉, 等. 神经干细胞移植治疗缺血性脑卒中研究进展[J]. 中风与神经疾病杂志, 2019,36(8), 36: 757-759. doi: 10.19845/j.cnki.zfysjjbzz.2019.08.023
2	BONAGUIDI M A, WHEELER M A, SHAPIRO J S, et al.In vivo clonal analysis reveals self-renewing and multipotent adult neural stem cell characteristics[J]. Cell, 2011, 145(7): 1142-1155. doi: 10.1016/j.cell.2011.05.024
3	MING G L, SONG H J. Adult neurogenesis in the mammalian brain: significant answers and significant questions[J]. Neuron, 2011, 70(4): 687-702. doi: 10.1016/j.neuron.2011.05.001
4	刘伟, 吴浩, 许鹏程. 成年哺乳动物海马齿状回神经发生的研究进展[J]. 山东医药, 2011, 51(9): 111-113. doi: 10.3969/j.issn.1002-266X.2011.09.071
5	DU W F, CAI H, WANG M Y, et al. Simultaneous determination of six active components in crude and processed Fructus Corni by high performance liquid chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis, 2008, 48(1): 194-197. doi: 10.1016/j.jpba.2008.04.021
6	曹岗, 邵玉蓝, 张云, 等. 山茱萸中马钱子苷的研究进展[J]. 现代药物与临床, 2009, 24(5): 272-275.
7	MATSUDA S, NAKAGAWA Y, AMANO K, et al. By using either endogenous or transplanted stem cells, which could you prefer for Neurogenesis?[J]. Neurogenesis Research,2018,13(10): 1731-1732. doi: 10.4103/1673-5374.238609
8	黄志, 张良明, 陈瑞强, 等. Purmorphamine对神经干细胞分化的影响及其机制[J]. 中国组织工程研究, 2018, 22(29): 4675-4680. doi: 10.3969/j.issn.2095-4344.0626
9	KASHEM M A, SULTANA N, BALCAR V J. Exposure of rat NSCs to ethanol affects cell numbers and alters of 28 proteins[J]. Neurochemical Research, 2018, 43(9): 1841-1854. doi: 10.1007/s11064-018-2600-1
10	ZHENG P D, MUNGUR R, ZHOU H J, et al. Ginkgolide B promotes the proliferation and differentiation of NSCs following cerebral ischemia/reperfusion injury, both in vivo and in vitro[J]. Neurogenesis Research. 2018; 13(7): 1204-1211. doi: 10.4103/1673-5374.232476
11	赵舒武, 蔡青, 王晓玲, 等. 补阳还五汤载药血清对缺氧性神经干细胞增殖和分化的影响[J]. 江苏中医药, 2013, 45(1): 73-75. doi: 10.3969/j.issn.1672-397X.2013.01.046
12	王文, 黄文婷, 艾厚喜, 等. 莫诺苷和马钱素对人神经母细胞瘤株SY5Y生长的影响[J]. 中国药理通讯, 2007(2): 13.

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Regulatory effects of Loganin on the proliferation, survival, and differentiation of nueral stem cells

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