Abstract:

    We established a preparation approach of certified reference material of glycyrrhizic acid. Glycyrrhizic acid was separated by silica gel column chromatography and recrystallization, whose chemical structure was denoted by UV, IR, MS and NMR. Its homogeneity and stability test were also systematically checked. Eight qualified domestic laboratories cooperatively certified its value. Results show that its certified value is 99.29%, and the expanded uncertainty with 95% confidence coefficient is 0.12%. The reference material satisfied the technical requirement of domestic certified reference material, so it could be used for the validation of glycyrrhizic acid detection method and quality control over relative products.

Key words: glycyrrhizic acid, certified reference material, homogeneity, stability, certification, uncertainty