Abstract:

      We employed pH-zone refining counter-current chromatography to prepare high-purity sinoacutine from Stephania kwangsiensis. Its solvent system was composed of petroleum ether-ethyl acetate-methanol water (3:7:1:9, V/V), in which triethylamine (10 mmol/L) was added to the upper organic stationary phase as a retainer and hydrochloric acid (10 mmol/L) was added to the aqueous mobile phase as an eluter. The structure of sinoautine was identified by ESI-MS,¹H NMR and ¹³C NMR. The purity of sinoacutine determined by RP-HPLC was 99.5%. This approach has such advantages as high-efficiency and high-purity, suitable for the preparation of large amount of sinoacutine.

Key words: Stephania kwangsiensis, Sinoacutine, pH-zone-refining counter-current chromatography