Abstract:

      We constructed recombinant plasmid pUT-M by ligating mpd gene on a suicide plasmid pUT-Km1. We integrated mpd gene onto the chromosome of B.vietnamiensis B418 by triparental hybridization and obtained a multifunctional engineering stain. Experimental results show that the engineering stain retains its original activity of phosphorus or potassium decomposition and nitrogen fixation. The strain degrades chlorpyrifos in solution at a rate of 65% 48 hours later. Moreover, the strain can also degrade chlorpyrifos in simulated soil and the degradation rate can reach 91% 20 days later.

Key words: chlorpyrifos degradation, multifunctional bacterium strain, construction of recombinant plasmid, Burkholderia vietnamiensis, soil bioremediation