Abstract:

       This paper is to establish an approach to separate highpurity reference material of solanesol from tobacoo leaves. We initially obtained crude reference material of solanesol with petroleum ether extraction, dynamic saponification and silica gel chromatography. We then employed preparative highperformance liquid chromatography whose ingredient ratio was 60% methanol and 40% ethanol to refine and purify the acquired crude reference material of solanesol. The purified product whose structure was identified with ESI-MS,IR,¹H-NMR and ¹³C-NMR and whose purity of 99.7% was determined with HPLC satisfied the requirement of reference material. Such an approach is simple, rapid and reproductive and its product has quite high purity so it is suitable for the preparation of reference material of solanesol.

Key words: waste tobacoo leaves, solanesol, preparation