Abstract:

         This paper presented a new method for isolation and culture of lichenized fungi and lichen endophyte fungi. We directly selected medulla mycelium for cultivation with PDA nutrition medium. The growth rate of the community of lichenized fungi was 80.43%, while the contamination rate was only 7.14% under the conditions of nutrition medium of 25℃ and 14 days later. The superiority of this method was therefore simplicity, highefficiency and low contamination rate.

Key words: lichenized fungi, lichen endophyte fungi, isolation and culture