土壤杆菌固氮、溶磷活性及对植物的促生作用

doi:10.3976/j.issn.1002-4026.2017.04.005

SHANDONG SCIENCE ›› 2017, Vol. 30 ›› Issue (4): 26-30.doi: 10.3976/j.issn.1002-4026.2017.04.005

• Agricultural Microbiology • Previous Articles Next Articles

Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium

WEI Yan-li¹, Maarten RYDER ², LI Ji-shun¹, LI Ling¹, YANG Yu-zhong¹, YANG He-tong^1,3*

1. Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250014, China; 2. School of Agriculture, Food and Wine, University of Adelaide, Adelaide 5064, Australia; 3.Research Center of Sustainable Development in the Yellow River Delta，Dongying 257000, China

Received:2017-05-11 Online:2017-08-20 Published:2017-08-20

Abstract

Abstract:

Through selective medium, the function of fixing nitrogen and dissolving phosphorus of 26 Agrobacterium strains were studied by both qualitative and quantitative analysis methods. Meanwhile, growthpromoting effect of Australian biocontrol strain K1026 on cucumber and wheat was measured by pot experiment. The results showed that all the strains regardless of pathogenicity or nonpathogenicity could grow normally on nitrogenfree medium. 19 of the 26 strains had the ability to dissolve phosphorus, the Phosphate released by K1026 in Pikovskaya broth medium was up to 109.11 μg/mL , having the strongest activities among all stains. The pot experiments showed that K1026 had obvious growthpromoting effect on the cucumber seedlings, and the root length and the dry weight of the root biomass was increased by 25.49%, 62.96%, respectively, compared with the control. However, K1026 had no obvious effect on the growthpromotion of wheat.

Key words: Agrobacterium rhizogenes K1026, phosphate-solubilisation, growth promotion effect, Agrobacterium tumefaciens, nitrogen fixation

CLC Number:

S436.629

WEI Yan-li, Maarten RYDER,LI Ji-shun, LI Ling, YANG Yu-zhong, YANG He-tong. Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium[J].SHANDONG SCIENCE, 2017, 30(4): 26-30.

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Nitrogen fixation, phosphate solubilizing activity and plant growth promotion of Agrobacterium

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