基于斑马鱼模型的琥珀酰缺端胶原蛋白功效研究

doi:10.3976/j.issn.1002-4026.2025092

摘要/Abstract

摘要： 本研究旨在系统评估琥珀酰缺端胶原蛋白在抗炎、抗氧化、美白及促进胶原分泌方面的综合功效。通过构建硫酸铜诱导的斑马鱼炎症模型，发现不同质量浓度的琥珀酰缺端胶原蛋白均可极显著抑制中性粒细胞趋化（P< 0.001），证明其具有抗炎活性。在4-叔丁基过氧化氢诱导的斑马鱼氧化应激模型中，25.00 μg/mL的琥珀酰缺端胶原蛋白可显著降低内源性活性氧水平（P< 0.001），表明其能通过清除自由基发挥抗氧化作用。同时，在该浓度下，该蛋白通过抑制黑色素生成表现出美白效果。进一步利用人真皮成纤维细胞实验显示，125.00 μg/mL的琥珀酰缺端胶原蛋白可促进I型胶原蛋白的分泌，提示其对皮肤修复具有潜在作用。综上，本研究证实琥珀酰缺端胶原蛋白在6.25–25.00 μg/mL浓度范围内兼具抗炎、抗氧化、美白及促进胶原生成的多种生物活性，为其在功能性护肤品或医药材料中的应用提供了实验依据。

关键词: 琥珀酰缺端胶原蛋白, 斑马鱼模型, 抗炎, 抗氧化, 美白

Abstract: This study aimed to systematically evaluate the anti-inflammatory, antioxidant, whitening, and collagen-promoting effects of succinylated telocollagen. In a copper sulfate-induced zebrafish inflammation model, succinylated telocollagen at varying concentrations significantly reduced neutrophil migration (P< 0.001), demonstrating potent anti-inflammatory activity. In a tert-butyl hydroperoxide-induced oxidative stress model, treatment with 25.00 μg/mL of succinylated telocollagen markedly decreased endogenous reactive oxygen species (ROS) levels, as indicated by reduced fluorescence intensity of 2′,7′-dichlorodihydrofluorescein (P< 0.001), confirming its antioxidant capacity. At the same concentration, the protein also exhibited whitening effects by inhibiting melanin production. Furthermore, in human dermal fibroblast cultures, a concentration of 125.00 μg/mL promoted the secretion of type I collagen, suggesting potential skin-repair benefits. These findings collectively indicate that succinylated telocollagen, within the concentration range of 6.25–25.00 μg/mL, possesses integrated anti-inflammatory, antioxidant, whitening, and collagen-enhancing properties, supporting its potential application in functional skincare and biomedical materials.

Key words: , succinoyl atelocollagen, zebrafish model, anti-inflammation, antioxidation, whitening

中图分类号:

R965.1

梁铭辉, 叶子康, 周宁欣, 崔洁, 孙立和, 彭新生. 基于斑马鱼模型的琥珀酰缺端胶原蛋白功效研究[J]. 山东科学, 0, (): 1-.

LIANG Minghui, YE Zikang, ZHOU Ningxin, CUI Jie, SUN Lihe, PENG Xinsheng. Research on the efficacy of succinoyl atelocollagen based on zebrafish models[J]. Shandong Science, 0, (): 1-.

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