基于斑马鱼模型对Betukladin降血脂活性与机制分析

doi:10.3976/j.issn.1002-4026.2025085

山东科学

• 药理与毒理 •

基于斑马鱼模型对Betukladin降血脂活性与机制分析

杜兴硕^a，展文^a，高松英^a，孙长高^b，徐明贤^b，张思晨^b，李洪亮^b，夏青^a，何秋霞^a,b*

齐鲁工业大学（山东省科学院）ａ.生物研究所山东省人类疾病斑马鱼模型与药物筛选工程技术研究中心；ｂ.高新技术产业（中试）基地，山东济南 250103

收稿日期:2025-08-20 接受日期:2025-09-24 上线日期:2026-01-20
通信作者: 何秋霞 E-mail:heqx@ sdas.org
作者简介:杜兴硕(2001—),女,硕士研究生,研究方向为药物筛选｡E-mail:xingshuo2001@163.com
基金资助:
山东省自然科学基金创新发展联合基金（ZR202306200045）；中央引导地方科技发展资金项目（YDZX2022169）

Analysis of the lipid-lowering activity and mechanism of Betukladin based on a zebrafish model

DU Xingshuo ^a, ZHAN Wen ^a, GAO Songying ^a, SUN Changgao ^b, XU Mingxian ^b, ZHANG Sichen ^b, LI Hongliang ^b, XIA Qing ^a, HE Qiuxia ^{a, b}*

(a. Shandong Provincial Engineering Research Center for Zebrafish Models of Human Diseases and Drug Screening, Institute of Biology;b. High-Tech Industry (Pilot Test) Base, Qilu University of Technology (Shandong Academy of Sciences),Jinan 250103, China)

Received:2025-08-20 Accepted:2025-09-24 Online:2026-01-20
Contact: HE Qiuxia E-mail:heqx@ sdas.org

摘要/Abstract

摘要： Betukladin（БЕТУКЛАДИН）是一种由驯鹿地衣粉末和白桦树皮提取物组成的膳食补充剂，具有调节血糖、降血脂、抗炎及抗凝血等多种药理活性。本研究探讨Betukladin的降血脂活性及作用机制，旨在验证其活性，并为开发安全、经济的天然降脂药物提供科学依据。基于斑马鱼高脂模型，发现Betukladin可以显著改善斑马鱼高脂造模后的油红O染色IOD值、甘油三酯含量及总胆固醇含量升高的情况，表明了其具有降血脂的活性。通过网络药理学分析其可能通过脂质和动脉粥样硬化、PPAR信号通路等多通路协同发挥降脂的作用。RT-qPCR结果显示，其可以调控脂质代谢相关基因apoA1、LDLR和ANGPTL3，脂肪酸合成与氧化基因SREBF1、FASN、PPARα、cpt1a，胆固醇代谢基因CYP7A1、HMGCR、ABCG5、ABCG8。

关键词: 高脂血症, Betukladin, 驯鹿地衣, 白桦树皮, 降血脂, 斑马鱼模型

Abstract: Betukladin is a dietary supplement composed of reindeer lichen powder and birch bark extract, possessing various pharmacological activities such as blood glucose regulation, lipid lowering, anti-inflammatory effects, and anticoagulation. This study investigates the lipid-lowering activity and underlying mechanism of Betukladin, aiming to validate its efficacy and provide a scientific basis for developing safe and economical natural lipid-lowering drugs. Using a zebrafish hyperlipidemia model, we found that Betukladin significantly improved the elevated Oil Red O staining integrated optical density values, triglyceride levels, and total cholesterol levels induced by high-fat modeling, indicating its lipid-lowering activity. Network pharmacology analysis suggested that Betukladin may exert its lipid-lowering effects through multiple coordinated pathways, including lipid and atherosclerosis pathways and the PPAR signaling pathway. RT-qPCR results showed that it can regulate lipid metabolism–related genes (apoA1, LDLR, and ANGPTL3), fatty acid synthesis and oxidation genes (SREBF1, FASN, PPARα, and cpt1a,), and cholesterol metabolism–related genes (CYP7A1, HMGCR, ABCG5, and ABCG8).

Key words: hyperlipidemia, Betukladin, reindeer lichen, birch bark, lipid lowering, zebrafish model

中图分类号:

R961

杜兴硕, 展文, 高松英, 孙长高, 徐明贤, 张思晨, 李洪亮, 夏青, 何秋霞. 基于斑马鱼模型对Betukladin降血脂活性与机制分析[J]. 山东科学, 0, (): 1-.

DU Xingshuo, ZHAN Wen, GAO Songying, SUN Changgao, XU Mingxian, ZHANG Sichen, LI Hongliang, XIA Qing, HE Qiuxia. Analysis of the lipid-lowering activity and mechanism of Betukladin based on a zebrafish model[J]. Shandong Science, 0, (): 1-.

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基于斑马鱼模型对Betukladin降血脂活性与机制分析

Analysis of the lipid-lowering activity and mechanism of Betukladin based on a zebrafish model

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