红果樫木叶木脂素类化学成分研究

doi:10.3976/j.issn.1002-4026.20230094

山东科学 ›› 2024, Vol. 37 ›› Issue (3): 10-17.doi: 10.3976/j.issn.1002-4026.20230094

红果樫木叶木脂素类化学成分研究

闫慧娇^1a^,^1b(), 陈雨洁², 宋祥云^1a^,^1b, 李恩霞^1a^,^1b, 耿岩玲^1a^,^1b^,^*()

1.齐鲁工业大学(山东省科学院)a.山东省分析测试中心山东省大型精密分析仪器应用技术重点实验室; b.药学院山东省高等学校天然药物活性成分研究重点实验室,山东济南 250014
2.山东省食品药品审评查验中心,山东济南 250014

收稿日期:2023-06-06 出版日期:2024-06-20 发布日期:2024-06-14
通信作者: *耿岩玲,女,副研究员,研究方向为中药与复方活性成分及标准样品研制。Tel:0531-82605319, E-mail: gengyanling@126.com
作者简介:闫慧娇(1984—),女,博士,副研究员,研究方向为天然产物分离纯化及活性。E-mail: yanhuijiao01@163.com
基金资助:
国家自然科学基金(32200311);济南市高校20条(202228020);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2022PX029)

Lignan constituents from the leaves of Dysoxylum gotadhora

YAN Huijiao^1a^,^1b(), CHEN Yujie², SONG Xiangyun^1a^,^1b, LI Enxia^1a^,^1b, GENG Yanling^1a^,^1b^,^*()

1. a.Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center; b. Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
2. Shandong Center for Food and Drug Evaluation and Inspection, Jinan 250014, China

Received:2023-06-06 Online:2024-06-20 Published:2024-06-14

摘要/Abstract

摘要：

研究红果樫木叶的化学成分, 运用大孔树脂、硅胶、Sephadex LH-20和半制备色谱等多种色谱技术从红果樫木叶70%乙醇提取物中分离得到4个木脂素类化合物。运用¹H NMR(核磁共振)、¹³C NMR和MS(质谱)波谱学数据等分别鉴定为4, 4'-dihydroxy-3, 3', 5, 5'-trtramethoxy-7, 9':7', 9-diepoxylignane(1), 4'-hydroxy-3, 4, 5, 3'-trtramethoxy-7, 9':7', 9-diepoxylignane(2), 4', 4″-dihydroxy-3, 3', 3″, 5, 5', 5″ -hexamethoxy-7, 9':7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineolignan-7″, 9″-diol(3)和4', 4″-dihydroxy-3, 3', 3″, 5, 5'-pentamethoxy-7, 9':7', 9-diepoxy-4, 8″-oxy-8, 8'-sesquineolignan-7″, 9″-diol(4)。化合物1~4均首次从樫木属植物中分离得到。使用DPPH(1, 1-二苯基-2-三硝基苯肼)自由基清除率测定化合物的抗氧化活性, 结果显示木脂素类化合物1~4具有良好的DPPH自由基清除能力。

关键词: 红果樫木, 化学成分, 结构鉴定

Abstract:

To study the constituents of the leaves of Dysoxylum gotadhora, the 70% ethanol extract was separated using macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 gel column chromatography, combined with semi-preparative HPLC and other methods. Four compounds 4,4'-dihydroxy-3,3',5,5'-trtramethoxy-7,9':7',9-diepoxylignane (1), 4'-hydroxy-3,4,5,3'-trtramethoxy-7,9':7',9-diepoxylignane (2), 4',4″-dihydroxy-3,3',3″,5,5',5″ -hexamethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineolignan-7″,9″-diol (3), 4',4″-dihydroxy-3,3',3″,5,5'-pentamethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineo lignan -7″,9″-diol (4) were isolated. Their structures were established by ¹H NMR, ¹³C NMR and MS spectroscopy techniques. Compounds 1 to 4 were obtained from the genus Dysoxylum for the first time. DPPH(1,1-diphenyl-2-picryhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) radical scavenging rate were used to determine the antioxidant activity. The results show that lignans have significant DPPH free radical scavenging ability.

Key words: Dysoxylum gotadhora, chemical constituents, structure identification

中图分类号:

R284.1

闫慧娇, 陈雨洁, 宋祥云, 李恩霞, 耿岩玲. 红果樫木叶木脂素类化学成分研究[J]. 山东科学, 2024, 37(3): 10-17.

YAN Huijiao, CHEN Yujie, SONG Xiangyun, LI Enxia, GENG Yanling. Lignan constituents from the leaves of Dysoxylum gotadhora[J]. Shandong Science, 2024, 37(3): 10-17.

图/表 6

参考文献 23

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红果樫木叶木脂素类化学成分研究

Lignan constituents from the leaves of Dysoxylum gotadhora

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